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从Lamiophlomis Rotata中提取,分离,结构识别和抗炎活性

Qibao Jiang¹, Mingming Hu¹, Xiaoge Li¹

¹National Key Laboratory of Chinese Medicine Modernization, Institute of Traditional Chinese Medicine, State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China.

International journal of biological macromolecules

|August 20, 2025

概括

这项研究详细介绍了从西藏药用植物Lamiophlomis rotata中提取三种多糖的结构分析. 这些化合物表现出抗炎性质,而化多糖体表现出增强的活性.

科学领域:

药理学和自然产品化学
药用植物研究
免疫学

背景情况:

一种西藏药用植物,具有显著的治疗潜力.
了解来自L. rotata的多糖是解锁其药用价值的关键.
关于L. rotata的特定多糖类的研究有限.

研究的目的:

从L. rotata空气部分提取,净化和识别三种多糖的结构.
研究这些多糖的体外抗炎作用.
评估化对L. rotata多糖的抗炎活性的影响.

主要方法:

从L. rotata中提取和净化多糖
使用分子量测定,单糖组成和甲基化分析进行结构阐明.
使用LPS诱导的RAW264.7细胞进行体外抗炎测定,包括氧化和细胞因子水平测量.
使用冰酸酸盐的纯化多糖体 (LHP2-2) 的化.

主要成果:

鉴定出具有不同分子量和复杂糖残留成分的三种多糖类 (LHP0-1,LHP2-1,LHP2-2).
这三种多糖都通过抑制氧化释放和降低iNOS,IL-6和TNF-α蛋白表达而表现出抗炎作用.
与非化形式相比,化LHP2- 2 (Se- LHP2- 2) 显示出增强的抗炎活性.

结论:

这项研究成功地鉴定了L. rotata中的三种新型多糖体.
旋转的多糖具有显著的抗炎性质.
化是一种有前途的策略,可以提高L. rotata多糖的治疗效果.

关键词:

抗炎作用旋转的多糖类化结构识别

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