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Updated: Sep 10, 2025

Method for Efficient Refolding and Purification of Chemoreceptor Ligand Binding Domain
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一个重新折叠的标签用于净化从大肠杆菌纳入体中的生物活性重组蛋白质

Bo Zhang1, Tong Wang1, Siyu Ni1

  • 1Jiangsu Key Laboratory of Brain Disease and Bioinformation, Research Center for Biochemistry and Molecular Biology, Xuzhou Medical University, Xuzhou 221004, China.

International journal of biological macromolecules
|August 20, 2025
PubMed
概括

一种新的67氨基酸融合标签P67显著增强了以大肠杆菌表达的不溶性重组蛋白的重新折叠. 这种蛋白质净化方法提高了生物活性蛋白的产量,也可以净化可溶性蛋白质.

关键词:
生物依赖的碳氧激酶.包容性机构是包容性的机构.这是一个P67标签.再组合蛋白质是一种重组蛋白质.再折叠可以重新折叠.

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Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
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