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Updated: Sep 10, 2025

NMR-Based Activity Assays for Determining Compound Inhibition, IC50 Values, Artifactual Activity, and Whole-Cell Activity of Nucleoside Ribohydrolases
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NMR-Based Activity Assays for Determining Compound Inhibition, IC50 Values, Artifactual Activity, and Whole-Cell Activity of Nucleoside Ribohydrolases

Published on: June 30, 2019

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伪乌里丁残留物作为血清核糖酶的基质

Clair S Gutierrez1, Bjarne Silkenath1, Volga Kojasoy1

  • 1Massachusetts Institute of Technology.

RNA (New York, N.Y.)
|August 20, 2025
PubMed
概括

在信使RNA (mRNA) 中,修改后的核酸伪尿素 (Ψ) 和N1-甲基伪尿素 (m1Ψ) 比未修改后的尿素 (U) 更能抵抗RNase A和RNase 1的酶分裂. 这种稳定性对于RNA疫苗开发至关重要.

关键词:
在X射线晶体学.酵素学 酵素学 酵素学诱导效应是一种诱导效应.分子动力学模拟,分子动力学模拟核酸2',3'-循环瓦纳酸.

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