Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

The Calvin Benson Cycle01:46

The Calvin Benson Cycle

4.7K
Ribulose 1,5- bisphosphate carboxylase/oxygenase (RuBisCo) is a critical enzyme that catalyzes carbon dioxide assimilation during photosynthesis. However, it is an inefficient enzyme, having an extremely slow catalytic rate. A typical enzyme can process about a thousand molecules per second; however, RuBisCo fixes only around three-carbon dioxides per second. Photosynthetic cells compensate for this slow rate by synthesizing very high amounts of RuBisCo, making it the most abundant single...
4.7K
The Supercomplexes in the Crista Membrane01:41

The Supercomplexes in the Crista Membrane

2.6K
The mitochondrial cristae membrane is the primary site for the oxidative phosphorylation (OXPHOS) process of energy conversion mediated through respiratory complexes I to V. These complexes have been widely studied for decades, and it has been proven that they form supramolecular structures called respiratory supercomplexes (SC). These higher-order complexes may be crucial in maintaining the biochemical structure and improving the physiological activity of the individual complexes while...
2.6K
The Inner Mitochondrial Membrane01:28

The Inner Mitochondrial Membrane

3.6K
The inner mitochondrial membrane is the primary site of ATP synthesis. The inner membrane domain that forms a smooth layer adjacent to the outer membrane is called the inner boundary membrane. This domain contains membrane transporters that drive metabolites in and out of the mitochondria.  In contrast, the inner membrane network that invaginates into the matrix space is called the cristae membrane. This domain accounts for principle mitochondrial function as it accommodates the protein...
3.6K
Carbon-dioxide Fixation01:28

Carbon-dioxide Fixation

84
Carbon dioxide fixation in prokaryotes enables the assimilation of inorganic carbon into organic molecules, supporting biosynthetic pathways, sustaining ecosystems, and contributing to the global carbon cycle. It also has industrial applications in carbon capture and bioproduct synthesis. Autotrophic organisms rely on this process to utilize CO₂ as a carbon source in diverse environments.The Calvin CycleThe Calvin cycle is the most widespread carbon fixation mechanism, primarily used by...
84
Cell Inclusions01:27

Cell Inclusions

196
Prokaryotic cells possess a variety of inclusions that play crucial roles in nutrient storage, metabolic processes, and environmental adaptation. These structures enable bacteria to thrive under fluctuating environmental conditions by storing essential resources and optimizing their metabolic efficiency.Carbon Storage: Poly-β-Hydroxybutyric Acid and Glycogen GranulesBacteria frequently store excess carbon in specialized granules. Poly-β-hydroxybutyric acid (PHB) granules are lipid...
196
Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

3.3K
3.3K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

A geometric criterion links HIV-1 capsid topography to its biophysical properties and function.

Nature communications·2026
Same author

Structural Dynamics of RNA Polymerase II During Nucleotide Addition Cycle.

bioRxiv : the preprint server for biology·2026
Same author

Structural Advances in Respiratory Syncytial Virus: Implications for Vaccine and Antiviral Development.

Microorganisms·2026
Same author

A geometric criterion links HIV-1 capsid topography to its biophysical properties and function.

bioRxiv : the preprint server for biology·2026
Same author

Spatial Relations between Coccoliths and Their Confining Membrane During Crystal Morphogenesis.

Journal of the American Chemical Society·2026
Same author

Lenacapavir prevents production of infectious HIV-1 by abrogating immature virus assembly.

bioRxiv : the preprint server for biology·2026

相关实验视频

Updated: Sep 10, 2025

Author Spotlight: Innovative Approaches to Understanding Plant Structure-Function Relationships for Climate-Resilient Crops
06:04

Author Spotlight: Innovative Approaches to Understanding Plant Structure-Function Relationships for Climate-Resilient Crops

Published on: July 12, 2024

1.1K

皮尔诺伊德Rubisco的细胞内结构和变异性

Nadav Elad1,2, Zhen Hou3, Maud Dumoux4

  • 1Department of Chemical Research Support, Weizmann Institute of Science, Rehovot, Israel.

Nature communications
|August 20, 2025
PubMed
概括
此摘要是机器生成的。

研究人员可视化了Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) 在Chlamydomonas reinhardtii. pyrenoid中的原生结构. 这揭示了鲁比斯科 (Rubisco).

更多相关视频

Evaluation of Photosynthetic Efficiency in Photorespiratory Mutants by Chlorophyll Fluorescence Analysis
10:46

Evaluation of Photosynthetic Efficiency in Photorespiratory Mutants by Chlorophyll Fluorescence Analysis

Published on: December 9, 2022

2.1K
Establishment of Microbial Eukaryotic Enrichment Cultures from a Chemically Stratified Antarctic Lake and Assessment of Carbon Fixation Potential
14:38

Establishment of Microbial Eukaryotic Enrichment Cultures from a Chemically Stratified Antarctic Lake and Assessment of Carbon Fixation Potential

Published on: April 20, 2012

11.5K

相关实验视频

Last Updated: Sep 10, 2025

Author Spotlight: Innovative Approaches to Understanding Plant Structure-Function Relationships for Climate-Resilient Crops
06:04

Author Spotlight: Innovative Approaches to Understanding Plant Structure-Function Relationships for Climate-Resilient Crops

Published on: July 12, 2024

1.1K
Evaluation of Photosynthetic Efficiency in Photorespiratory Mutants by Chlorophyll Fluorescence Analysis
10:46

Evaluation of Photosynthetic Efficiency in Photorespiratory Mutants by Chlorophyll Fluorescence Analysis

Published on: December 9, 2022

2.1K
Establishment of Microbial Eukaryotic Enrichment Cultures from a Chemically Stratified Antarctic Lake and Assessment of Carbon Fixation Potential
14:38

Establishment of Microbial Eukaryotic Enrichment Cultures from a Chemically Stratified Antarctic Lake and Assessment of Carbon Fixation Potential

Published on: April 20, 2012

11.5K

科学领域:

  • 结构生物学 结构生物学
  • 生物化学 生化学
  • 藻类生物学 藻类生物学

背景情况:

  • 利布洛斯-1,5-双酸碳氧化酶/氧化酶 (Rubisco) 对于全球二氧化碳 (CO2) 固定至关重要.
  • 在真核藻类中,体有机体集中了二氧化碳,以提高鲁比斯科的催化效率.
  • 鲁比斯科的体外结构是众所周知的,但其在体内的原生状态在很大程度上是未知的.

研究的目的:

  • 为了确定Clamydomonas reinhardtii.pyrenoid中的Rubisco的本土结构和动态.
  • 研究鲁比斯科在其自然细胞环境中的结构变化和相互作用.
  • 了解Rubisco的功能组织,以改善二氧化碳的固定.

主要方法:

  • 低温电子断层扫描被用来对细胞进行成像.
  • 用冷聚焦离子束削用于样品制备.
  • 应用了子图平均值来重建本土的鲁比斯科结构.

主要成果:

  • 在pyrenoid中确定了Rubisco的多个结构子集.
  • 在最高分辨率的地图中,Rubisco被观察到在一个活跃的构造中.
  • 在活性部位,二元接口和结合蛋白接触区域发现了显著的局部变异.

结论:

  • 这项研究提供了对原生Rubisco结构和藻类pyrenoid内部动态的全面了解.
  • 这些发现揭示了Rubisco的局部结构异质性,影响了其功能.
  • 对Rubisco组织的洞察力为增强二氧化碳固定提供了宝贵的视角.