不活化的环境细菌分离物及其内的免疫刺激活性
Max Dekeukeleire1,2, Dieter Vandenheuvel1, Tippapron Khondee1
1Laboratory of Applied Microbiology and Biotechnology (LAMB), Department of Bioscience Engineering, University of Antwerp, Antwerp, Belgium.
Scientific reports
|August 20, 2025
概括
环境细菌,包括子和植物细胞,激活人类的免疫反应. 与甲相比,热/UV-C 无活化保留了细菌形态,并增强了免疫激活,揭示了菌株特定的免疫相互作用.
科学领域:
- 微生物学 微生物学
- 免疫学 免疫学 免疫学
- 细菌学 细菌学是一门学科.
背景情况:
- 细菌物种由于子形成而具有环境持久性.
- 环境细菌与人体免疫系统之间的相互作用需要进一步研究.
- 了解这些相互作用对于健康和工业应用至关重要.
研究的目的:
- 为了研究环境中Bacillus分离物的免疫刺激潜力.
- 优化Bacillus的无活化方法,同时保持免疫刺激活性.
- 为了阐明细菌诱导的免疫激活机制.
主要方法:
- 测试了七种环境中的细菌分离物 (B. subtilis,B. velezensis,B. licheniformis,B. pumilus).
- 通过热/UV-C和甲来评估无活化,通过扫描电子显微镜评估形态.
- 使用人类记者细胞测定 (THP1-Dual,HEK-Blue hTLR2/6,hTLR4) 评估免疫激活.
主要成果:
- 热/UV-C处理保留了Bacillus形态,并增强了免疫活性,比甲素更多.
- 子和植物细胞都激活了单细胞中的免疫转录因子 (NF-κB,IRF).
- 子诱导的免疫反应低于植物性细胞 (1.5到8倍),与菌株/物种特定的变化.
- 细菌分离物激活了TLR2/6,但没有TLR4;子包裹组件可能解释了反应差异.
结论:
- 环境细菌分离物具有免疫刺激的潜力.
- 热/UV-C是一种合适的无活化方法,可以保持Bacillus的免疫激活特性.
- 细菌 - 人类免疫相互作用是复杂的,取决于菌株和物种,并通过TLR2/6.6进行调解.
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