在CgDbf2/CgMob1-CgCdc14酸化模块中介的酸酶活性调节了Collettotrichum gloeosporioides中的MEN信号和致病性
Jiyun Yang1, Mei-Ling Sun1, Yu-Ting Pan1
1Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, China.
Plant, cell & environment
|August 21, 2025
概括
涉及CgDbf2/CgMob1和CgCdc14的真菌线性退出网络 (MEN) 途径通过协调细胞壁完整性和反应性氧物种反应,对植物感染至关重要.
科学领域:
- 菌群学
- 分子植物病理学
- 细胞生物学
背景情况:
- 线性出口网络 (MEN) 途径调节细胞形态和真核生物的致病性.
- 连接MEN信号的机制,细胞壁完整性 (CWI),宿主反应性氧物种 (ROS) 稳定性和植物病原菌的毒性是不清楚的.
研究的目的:
- 阐明MEN通路,特别是CgDbf2和CgMob1在植物病原菌Colletotrichum gloeosporioides的毒性中的作用.
- 研究CgDbf2,CgMob1和酸酶CgCdc14在调节真菌病原性的相互作用.
主要方法:
- RNA测序 (RNA-seq) 的方法
- 生物化学分析
- 基因分析
- 蛋白质复合体的识别
- 酸化地点的测绘
主要成果:
- CgDbf2和CgMob1在C. gloeosporioides中形成一个关键的复合体,对细胞壁完整性 (CWI) 和防真菌剂耐药性至关重要.
- 由于压缩体形成和侵入性生长的缺陷,CgDBF2或CgMOB1的破坏严重损害了毒性.
- 在Ser427和Thr428中化CgCdc2,增强其酸酶活性并导致毒性.
- CgDbf2/CgMob1-CgCdc14轴集成了CWI和ROS反应,这对植物感染至关重要.
结论:
- CgDbf2/CgMob1复合体对于C. gloeosporioides的毒性至关重要,它调解了CWI和真菌杀菌剂的耐药性.
- 通过CgDbf2对CgCdc14的酸化对真菌病原性至关重要,突出显示了MEN和CWI之间的交叉声.
- 这项研究为真菌毒性机制和潜在的抗真菌点提供了新的见解.
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