菌根液体染色法测定莫西弗洛克萨及其杂质
Alma Salkić1, Mira Zečević2, Biljana Otašević3
1ZADA Pharmaceuticals d.o.o. Bistarac Donji bb, 75300 Lukavac, Bosnia and Herzegovina Faculty of Pharmacy, University of Tuzla, Studentska 6, 75000 Tuzla, Bosnia and Herzegovina. alma.avdic.salkic@zada.ba.
开发了一种新的环保状高性能液体染色学 (HPLC) 方法来分析莫西素及其杂质. 这种绿色分析方法为药物分析提供了卓越和可持续的方法.
科学领域:
- 分析化学
- 绿色化学
背景情况:
- 莫西弗洛克萨分析需要选择性和环保的方法.
- 现有的方法可能无法充分检测相关物质和降解产品.
研究的目的:
- 开发和优化针对莫西素及其相关物质的选择性,环保的小细胞HPLC方法.
- 验证开发的方法是否适用于药物分析.
主要方法:
- 微粒高性能液体染色学 (HPLC) 在C18柱上进行同位素化.
- 使用中央复合设计进行优化.
- 移动阶段:含二酸,二硫酸 (SDS) 和的可生物降解水溶液.
- 检测在295nm,流量为0.6ml/min,注射体积为5μl,温度为60°C.
主要成果:
- 成功开发了一种选择性和环保的微粒 HPLC 方法.
- 该方法证明适合量化药物中的莫西素和相关物质.
- 绿色分析方法指数 (GAPI) 证实了该方法的环境优势.
结论:
- 开发的微粒HPLC方法对莫西素及其杂质的分析是有效的.
- 该方法符合绿色化学原则,提供可持续的分析解决方案.
- 这种经过验证的程序适用于moxifloxacin药物质量控制.
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