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通过膜脂质重塑和差异性蛋白质表达,冷应激增强了乳酸菌B6的冷耐性
Ting Yang1, Jia Zhang1,2, Ranjin Zhang1,2
1State Key Laboratory of Dairy Biotechnology, Shanghai Engineering Research Center of Dairy Biotechnology, Dairy Research Institute, Bright Dairy & Food Co., Ltd., Shanghai 201103, China.
Current research in microbial sciences
|August 21, 2025
概括
寒冷压力增加了乳酸细菌的冷耐受性. Lacticaseibacillus rhamnosus B6通过动态脂质组重塑和蛋白质调节提高了生存率,这对益生菌和初始培养的生存能力至关重要.
科学领域:
- 微生物学
- 食品科学
- 生物化学
背景情况:
- 乳酸细菌的冷耐受性对于食品加工至关重要.
- 了解分子机制可以提高益生菌和初始培养的保存.
研究的目的:
- 研究Lacticaseibacillus rhamnosus菌株的冷凍耐受性的分子機制.
- 评估冷压预处理对细菌活力的影响.
主要方法:
- 在液冷之前,在4°C处预处理Lacticaseibacillus rhamnosus B6和KF7.
- 使用SEM和量化膜脂肪酸成分评估细胞存活率.
- 使用蛋白质组分析识别差异表达的蛋白质.
主要成果:
- 与KF7 (30%) 和对照组相比,冷压B6的存活率显著提高 (53%).
- 寒冷压力增加了B6膜中的不和脂肪酸 (UFA) 和UFA/SFA比率.
- 参与脂肪酸生物合成,转化和运输的219种蛋白质在冷压B6中得到了上调.
结论:
- L. rhamnosus B6 具有双重的冷耐受机制,涉及脂层重塑和蛋白质调节.
- 细胞表面特征和冷应激对于在深过程中增强益生菌和初始培养的活力至关重要.
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