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Ligand Binding Sites

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Proteins are dynamic macromolecules that carry out a wide variety of essential processes; however, the activities of most proteins depend on their interactions with other molecules or ions, known as ligands.
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Synthetic biology is an interdisciplinary science that involves using principles from disciplines such as engineering, molecular biology, cell biology, and systems biology. It involves remodeling existing organisms from nature or constructing completely new synthetic organisms for applications such as protein or enzyme production, bioremediation, value-added macromolecule production, and the addition of desirable traits to crops, to name a few.
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The cultivation of environmental microorganisms has long been hindered by the inability to replicate complex native conditions in vitro. The isolation chip (iChip) addresses this limitation by facilitating the growth of previously uncultivable microorganisms through in situ incubation. Designed for high-throughput microbial cultivation, the iChip comprises hundreds of microchambers, each capable of housing a single microbial cell. These microchambers are loaded with a mixture of molten agar and...
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Chemoselective Modification of Viral Surfaces via Bioorthogonal Click Chemistry
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细胞微环境的生物直角工程

Ping Zhou1, Lauren Brown2, Christopher M Madl1

  • 1Department of Materials Science & Engineering, University of Pennsylvania, Philadelphia, PA 19104, USA.

Advanced functional materials
|August 21, 2025
PubMed
概括

新的生物直角异铁结合为组织工程提供先进的水凝交叉连接. 这扩大了材料特性控制,使细胞行为和长期细胞培养的精确调节成为可能.

科学领域:

  • 生物材料科学
  • 组织工程
  • 聚合物化学

背景情况:

  • 水凝是组织工程中的关键支架,
  • 目前的交叉连接方法通常涉及反应性条件,限制对材料特性和细胞命运的控制.
  • 现有的凝形成的生物直角化学反应是有限的,限制了材料特性的独立调.

研究的目的:

  • 引入异铁连接作为凝交叉连接的新生物对等策略.
  • 扩大可用的生物直角工具包,以精确控制水凝的特性.
  • 开发用于再生医学的先进水凝.

主要方法:

  • 研究了与氧胺 (ChO),四氨酸 (Tz) 和亚甲胺 (AMI) 功能组的异酸结合.
  • 合成的聚乙烯糖醇 (PEG) 水凝使用异二结合.
  • 结合ChO功能化的PEG与异基功能化的弹性蛋白 (ELP).
  • 评估水凝特性,包括凝动力学,机械稳定性和生物相容性.
  • 评估了以酸循环添加的异酸-ChO结合的正交性.

主要成果:

  • 使用异酸-ChO,异酸-Tz和异酸-AMI结合证明了水凝的形成.
  • 异二结合表现出最佳的凝动力学和机械性能.
关键词:
生物对角化学工程外细胞基质水凝异尼结合重组蛋白质材料

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  • 通过异二结合交联的PEG水凝显示出快速凝,弹性,稳定性和生物相容性.
  • 通过使用PEG- ELP水凝来调节细胞扩散,同时控制网络连接和连接物呈现.
  • 证实了异-ChO结合到阿酸-循环添加的正交性,以实现独立的水凝功能化.

    • 结论:

    • 异酸连接是水凝交叉连接的多功能和生物相容平台.
    • 异二结合提供了一种强大的方法来制造具有可调节性质的先进水凝.
    • 这些水凝支持长期培养各种人体细胞类型用于再生医学.
    • 证明的直角性允许在活细胞环境中对水凝进行连续的生物直角性修饰.