通过TNF-α-Primed外分细胞抑制通过miR-146a-5p/TXNIP/NLRP3轴加速T2DM伤口愈合
Jiaqi Li1,2,3, Xiaoxuan Lin4, Jinyang Wang1,2,3
1Hospital of Stomatology, Guanghua School of Stomatology, Sun Yat-Sen University, Guangzhou, 510055, People's Republic of China.
International journal of nanomedicine
|August 21, 2025
概括
通过调节树突细胞功能,来自脂肪干细胞 (T-exos) 的TNF-α预条件外体改善了2型糖尿病的伤口愈合. 这些T-exos通过miR-146a-5p/ TXNIP/ NLRP3途径抑制炎症并促进组织修复.
科学领域:
- 免疫学
- 复原医学
- 生物技术
背景情况:
- 由于高血糖引起的免疫功能障碍,二型糖尿病 (T2DM) 会影响伤口愈合.
- 树突细胞 (DCs) 对于皮肤伤口愈合至关重要,但高葡萄糖环境会对其产生负面影响.
- 介质干细胞衍生外体 (MSC-exos) 显示出免疫调节潜力,脂肪衍生外体 (ADSC) 很容易获得.
研究的目的:
- 研究TNF-α预先条件的ADSC-exos (T-exos) 在恢复DC功能和加速T2DM中伤口愈合的治疗潜力.
- 阐明T-exos在调节DC活动和促进糖尿病伤口修复中的潜在分子机制.
主要方法:
- 从TNF-α治疗的ADSC中分离T-exos.
- 在高葡萄糖条件下,T-exos对DC免疫反应的影响的评估.
- 在T2DM小鼠模型中评估T-exos在促进伤口关闭方面的有效性.
- 对miR-146a-5p/TXNIP/NLRP3信号通路的研究.
主要成果:
- 在高葡萄糖条件下,T-exos抑制了DC激活和NLRP3炎症酶活性.
- 在体内,T- exos显著加快了T2DM小鼠的伤口关闭,增强了原沉积,血管生成和纤维细胞增殖.
- 在机理上,T-exos丰富了miR-146a-5p,它针对TXNIP抑制了DC中的NLRP3炎症酶激活,这种效果取决于miR-146a-5p.
结论:
- 通过miR-146a-5p/TXNIP/NLRP3轴,T-exos有效调节直流激活,调节NLRP3炎症体通路.
- 通过减少炎症和促进组织修复,T-exos在治疗T2DM相关的伤口方面具有显著的临床应用潜力.
更多相关视频
11:34A Simple and Efficient Method for Testing Immunomodulatory Agents for Generation of Tolerogenic Dendritic Cells from Human CD14+ Monocytes
Published on: April 11, 2025
421
13:36Author Spotlight: Dendritic Cells Maturation Using Sialidases-Based Enzymatic Treatment of the Cell Surface
Published on: October 20, 2023
3.6K
相关概念视频
01:24T Cell Types and Functions
1.4K
When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
1.4K
02:17The Tumor Microenvironment
6.8K
Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...
6.8K