介酶干细胞衍生 lncRNAs NKILA 通过脂肪酸氧化在胃癌中促进干性和化学抵抗
Xiao-Juan Lyu1, Lin Zhou2, Xu-Mian Jiang3
1Department of Oncology, The Central Hospital of Wuhan, Tongji Medical College, Wuhan 430024, Hubei Province, China.
World journal of gastrointestinal oncology
|August 21, 2025
概括
通过分泌 lncRNA NKILA,中酶干细胞 (MSC) 增强了胃癌 (GC) 的干性和化学抵抗力. 该分子通过miR-485-5p/STAT3途径促进脂肪酸氧化,为GC治疗提供潜在的治疗点.
科学领域:
- 癌症学
- 细胞生物学
- 分子生物学
背景情况:
- 胃癌 (GC) 是一个严重的全球健康挑战,生存率很低,特别是在晚期.
- 目前的手术和化疗疗法效果有限,
研究的目的:
- 研究介质干细胞 (MSC) 衍生长非编码RNA (lncRNA) NKILA在胃癌 (GC) 干细胞性和化学抵抗性的作用.
- 阐明涉及脂肪酸氧化和miR-485-5p/STAT3通路的机制.
主要方法:
- 与人类骨髓衍生的MSC一起培养GC细胞系 (AGS,MKN45).
- 评估干度,化学抵抗,脂肪酸氧化和ATP水平.
- 基因和蛋白质表达的分析使用qPCR,西方抹杀和露西法酶记者测定.
- 使用临床GC组织样本进行验证.
主要成果:
- 这些MSC增强了GC细胞干性,化学阻力和脂肪酸氧化.
- 通过对STAT3进行上调,MSC衍生 lncRNA NKILA促进了干性和化学抵抗性.
- 发现NKILA可调节miR-485-5p/ STAT3轴,增加能量代谢并支持癌症干细胞的特性.
结论:
- 通过分泌的 lncRNA NKILA,MSCs促进了 GC 的干性和化学抵抗性.
- NKILA增强脂肪酸氧化和STAT3激活,这对于癌症干细胞的维持至关重要.
- 针对NKILA/miR-485-5p/STAT3途径提供了一个有前途的治疗途径,以克服GC化学抵抗.
相关概念视频
lncRNA - Long Non-coding RNAs
8.9K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
8.9K
Cancer Stem Cells and Tumor Maintenance
5.0K
Early diagnosis and treatment can often cure cancer. However, even with treatment, residual cells called cancer stem cells (CSC) might remain, often causing tumor recurrence. These cancer stem cells possess the potential for self-renewal and multi-lineage differentiation and are often responsible for the therapeutic resistance displayed in most cancers.
Cancer stem cells are thought to originate from tissue-specific normal stem cells or progenitor cells. The normal stem cells usually reside in...
Cancer stem cells are thought to originate from tissue-specific normal stem cells or progenitor cells. The normal stem cells usually reside in...
5.0K
Formation of Muscle Fibers from Myoblasts
5.1K
De novo myogenesis, or the formation of muscle fibers, begins during the early embryonic stages. The skeletal muscle is formed from somites– blocks of embryonic cell layers. The somites are further divided into dermatomes, myotomes, sclerotomes, and syndetomes. Among these, the myotomes give rise to muscle fibers.
Muscle progenitor cells (MPCs) are formed from the myotomes. MPCs express genes that encode the transcription factors Pax3 and Pax7. Along with Pax 3/7, other transcription...
Muscle progenitor cells (MPCs) are formed from the myotomes. MPCs express genes that encode the transcription factors Pax3 and Pax7. Along with Pax 3/7, other transcription...
5.1K
Stem Cell Niche
5.3K
The stem cell niche is the dynamic microenvironment where stem cells reside. Inside these niches, the cells may remain undifferentiated, undergo high self-renewal, or become lineage-specific progenitors. Stem cells coexist with other niche cells, such as stromal cells. They also interact closely with the ECM. Cell-cell and cell-matrix communication occur via adhesion molecules or soluble factors that signal the stem cells and determine their fate. Stromal cells also provide survival signals to...
5.3K
mTOR Signaling and Cancer Progression
3.9K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
The mTOR pathway or the...
3.9K
Role Of Notch Signalling In Intestinal Stem Cell Renewal
2.2K
Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
2.2K


