来自植物的线粒体ATP合成二分体的亚断层平均值显示在外围茎上保留了额外的密度
Thorsten B Blum1, Karen M Davies1, Werner Kühlbrandt1
1Department of Structural Biology, Max Planck Institute of Biophysics, Max von Laue Strasse 3, 60438 Frankfurt am Main, Germany.
IUCrJ
|August 21, 2025
概括
植物线粒体ATP合成二极体形成独特的排列并表现出更大的灵活性. 在外围茎上观察到额外的密度,与酵母和其他生物不同.
科学领域:
- 线粒体生物学
- 结构生物学
- 生物化学
背景情况:
- ATP合成酶对于细胞能量产生至关重要.
- 线粒体结构,特别是晶状体,影响ATP合成酶的功能.
- 在各种生物体中观察到ATP合成酶的二元化,但其结构基础和影响尚未完全理解.
研究的目的:
- 研究植物线粒体中的ATP合成二元体的结构特征.
- 将植物ATP合成二元结构与其他模型生物的结构进行比较.
- 确定植物ATP合成二次体的任何独特结构特征.
主要方法:
- 从四种植物中净化线粒体.
- 低温电子断层扫描和亚断层扫描用于高分辨率的结构分析.
- 与Saccharomyces cerevisiae和其他已知的ATP合成酶结构进行比较的结构分析.
主要成果:
- 植物线粒体中的ATP合成二极体沿着曲的脊形成广泛的排列.
- 在植物ATP合成中发现了外围茎尖的新增密度,这种密度在Saccharomyces cerevisiae中不存在.
- 与酵母和哺乳动物相比,植物ATP合成二次体具有更大的二次角,表明更大的灵活性.
结论:
- 植物线粒体ATP合成酶具有独特的结构特征,包括额外的茎密度.
- 在植物ATP合成二元角度中观察到的灵活性可能与它们在曲线上的排列有关.
- 这些发现提供了植物ATP合成酶的结构多样性和功能适应性的见解.
相关概念视频
ATP Synthase: Mechanism
15.1K
In animals, the mitochondrial F1F0 ATP synthase is the key protein that synthesizes ATP molecules through a complex catalytic mechanism. While the nuclear genome encodes the majority of ATP synthase subunits, the mitochondrial genome encodes some of the enzyme's most critical components. The formation of this multi-subunit enzyme is a complex multi-step process regulated at the level of transcription, translation, and assembly. Defects in one or more of these steps can result in decreased...
15.1K
ATP Synthase: Structure
13.0K
ATP synthase or ATPase is among the most conserved proteins found in bacteria, mammals, and plants. This enzyme can catalyze a forward reaction in response to the electrochemical gradient, producing ATP from ADP and inorganic phosphate. ATP synthase can also work in a reverse direction by hydrolyzing ATP and generating an electrochemical gradient. Different forms of ATP synthases have evolved special features to meet the specific demands of the cell. Based on their specific feature, ATP...
13.0K
ATP Driven Pumps I: An Overview
8.5K
ATP-driven pumps, also known as transport ATPases, are integral membrane proteins. They have binding sites for ATP located on the membrane's cytosolic side and the ion-conducting domain in the transmembrane region. These pumps use the free energy released from ATP hydrolysis to move the solutes across cell membranes against an electrochemical gradient.
There are four main types of ATP-driven pumps - P-type, V-type, F-type, and ABC transporter. All these pumps are of varying complexities and...
There are four main types of ATP-driven pumps - P-type, V-type, F-type, and ABC transporter. All these pumps are of varying complexities and...
8.5K
ATP Yield
71.5K
Cellular respiration produces 30 - 32 ATP per glucose molecule. Although most of the ATP results from oxidative phosphorylation and the electron transport chain (ETC), 4 ATP are gained beforehand (2 from glycolysis and 2 from the citric acid cycle).
The ETC is embedded in the inner mitochondrial membrane and is comprised of four main protein complexes and an ATP synthase. NADH and FADH2 pass electrons to these complexes, which pump protons into the intermembrane space. This distribution of...
The ETC is embedded in the inner mitochondrial membrane and is comprised of four main protein complexes and an ATP synthase. NADH and FADH2 pass electrons to these complexes, which pump protons into the intermembrane space. This distribution of...
71.5K
Chemiosmosis and ATP Synthesis
222
The electron transport chain is a critical component of cellular respiration, occurring in the inner mitochondrial membrane. It facilitates the transfer of high-energy electrons from reduced cofactors NADH and FADH₂ to molecular oxygen, the final electron acceptor. This transfer of electrons through a series of protein complexes is tightly coupled to the translocation of protons across the membrane, generating a proton gradient essential for ATP synthesis.Electron Flow and Proton...
222
Energy to Drive Translocation
2.1K
Mitochondrial protein import is powered by two distinct energy sources: ATP hydrolysis and electrochemical potential across the inner membrane. Newly synthesized precursors are bound by cytosolic chaperones of the Hsp70 family, which guide them to the import receptors on the mitochondrial surface. Utilizing the energy of ATP hydrolysis, Hsp70 chaperones transfer these precursors to the TOM receptors on the mitochondrial outer membrane.
Generally, polypeptides are unfolded by two distinct...
Generally, polypeptides are unfolded by two distinct...
2.1K


