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Updated: Sep 10, 2025

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在Phenacoccus manihoti Matile-Ferrero (Hemiptera: Pseudococcidae) 中进行准确的qPCR规范化的基因鉴定和评估

A Sunny Rao1,2, K V Nagarjuna Reddy1,3, Gandhi Gracy Ramasamy4

  • 1Division of Genomic Resources, ICAR-National Bureau of Agricultural Insect Resources, Bangalore, 560024, Karnataka, India.

Molecular biology reports
|August 21, 2025
PubMed
概括

No abstract available in PubMed .

关键词:
松果虫规范化参考基因dsRNA 的时间在QPCR中

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Real Time RT-PCR02:57

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Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
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