结直肠癌中与RiboSis相关的基因表达的综合分析:对预后和免疫治疗的影响
Wei Song1, Min Zhou2, Yatao Wang1
1Department of Gastrointestinal Surgery, Renmin Hospital of Wuhan University, No. 238, Jiefang Road, Wuhan, 430060, Hubei, China.
Apoptosis : an international journal on programmed cell death
|August 21, 2025
概括
这项研究确定了与核糖体生物发生相关的基因 (RRG) 作为结直肠癌 (CRC) 的关键参与者. 使用这些基因的新预后模型准确地预测了结果,并提出了针对性免疫疗法的可能性.
科学领域:
- 癌症学
- 分子生物学
- 生物信息学
背景情况:
- 结肠直肠癌 (CRC) 是一个重大的全球健康挑战,需要新的生物标志物和治疗策略.
- 与核糖体生物发生 (RiboSis) 相关的基因 (RRG) 在细胞过程和癌症发育中起着至关重要的作用.
- 了解RRG在CRC中的作用对于改善患者结果和开发个性化治疗至关重要.
研究的目的:
- 研究RRG在结直肠癌中的重要性及其临床影响.
- 使用机器学习开发基于RiboSis相关基因的CRC预后特征.
- 在CRC中探索RiboSis状态,免疫微环境和治疗反应之间的关系.
主要方法:
- 使用来自CRC组织的单细胞RNA测序 (scRNA-seq) 数据.
- 使用非负矩阵因子 (NMF) 进行细胞亚型分类和非劣质性 (InferCNV) 进行恶性细胞识别.
- 在多个数据集中使用集成机器学习方法开发和验证预测签名 (TCGA,GSE17536,GSE39582).
主要成果:
- 鉴定出295个与CRC存活显著相关的不同表达的RiboSis相关基因 (DERRGs).
- 开发了一种经过验证的与RiboSis相关的预测模型,其预测准确度很高.
- 发现低的RiboSis签名得分与减少的免疫逃避和增强的免疫治疗反应相关,PFDN2被确定为关键的枢纽基因.
结论:
- 与核糖体生物发生相关的基因作为结直肠癌预后的有希望的生物标志物.
- 开发的预测模型为个性化CRC管理提供了有价值的工具.
- RRG,特别是PFDN2,是增强CRC治疗策略的潜在治疗点,包括免疫治疗.
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