细菌脂多糖和口腔状细胞癌之间的潜在关系的生物信息学分析和实验验证
Wannan Gao1, Hongyan Yuan2, Song Qing1
1College of Stomatology, North Sichuan Medical College, Affiliated Hospital of North Sichuan Medical College, Nanchong, China.
PloS one
|August 21, 2025
概括
这项研究显示,细菌脂多糖 (LPS) 通过与炎症相关的基因促进口腔状细胞癌 (OSCC). 五个关键的与LPS相关的枢纽基因 (LRHGs) 被确定为潜在的诊断生物标志物和OSCC的治疗点.
科学领域:
- 口腔瘤学
- 细菌病变
- 癌症研究中的生物信息学
背景情况:
- 包括脂多糖 (LPS) 在内的细菌毒性因子与瘤的发展有关.
- 在口腔状细胞癌 (OSCC) 中LPS的具体作用尚不清楚.
研究的目的:
- 使用与LPS相关的宿主基因研究口腔细菌在OSCC中的作用和机制.
- 确定OSCC的潜在诊断生物标志物和治疗点.
主要方法:
- 对OSCC数据集进行生物信息分析,以确定与LPS相关的差异表达基因 (LR-DEG).
- 基因本体学 (GO) 和基因和基因组的京都百科全书 (KEGG) 丰富分析.
- 蛋白与蛋白相互作用 (PPI) 网络分析以确定与LPS相关的枢纽基因 (LRHGs).
- 使用qRT-PCR和ROC分析验证LRHG.
主要成果:
- 确定了345个LR- DEG,主要与炎症相关的途径如细胞因子- 细胞因子受体相互作用和IL-17信号传递.
- 五种LRHG (Cxcl8,Cxcl10,Il- 1β,Il- 6,Mmp9) 被确定为潜在的OSCC诊断生物标志物,其AUC> 0. 7.
- 在OSCC中,LRHG被上调,与免疫细胞透相关,这表明了治疗潜力.
- 细菌LPS通过炎症介导途径在OSCC的进展中发挥着重要作用.
结论:
- 通过口腔细菌和LPS驱动的OSCC病变的新见解.
- 已识别的LRHG对于OSCC诊断至关重要,并为新疗法提供潜在的点.
- 这项研究强调了细菌LPS,炎症和OSCC发展之间的复杂联系.
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