Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Gastrulation01:56

Gastrulation

53.0K
Gastrulation establishes the three primary tissues of an embryo: the ectoderm, mesoderm, and endoderm. This developmental process relies on a series of intricate cellular movements, which in humans transforms a flat, “bilaminar disc” composed of two cell sheets into a three-tiered structure. In the resulting embryo, the endoderm serves as the bottom layer, and stacked directly above it is the intermediate mesoderm, and then the uppermost ectoderm. Respectively, these tissue strata...
53.0K
Cellular Differentiation00:57

Cellular Differentiation

5.7K
How does a complex organism such as a human develop from a single cell? It all starts from a single fertilized egg which gives rise to a vast array of cell types, such as nerve cells, muscle cells, and epithelial cells that characterize the adult? Throughout development and adulthood, cellular differentiation leads cells to assume their final morphology and physiology. Differentiation is the process by which unspecialized cells become specialized to carry out distinct functions.
A zygote is a...
5.7K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Redefining light-chain smoldering multiple myeloma: prevalence and progression risk.

Leukemia·2026
Same author

Exploring the Functional Impact of Individual <i>DDX41</i> Variants With a Fast and Robust Cell-Based Method.

Human mutation·2026
Same author

Transcriptional repression by TGIF2 coordinates neurogenic priming and neural stem cell maintenance.

Science advances·2026
Same author

Mosaic Chromosomal Alterations Identify Ultra-High Risk Clonal Hematopoiesis in Patients With Lymphoma Undergoing Intensive Chemotherapy.

American journal of hematology·2026
Same author

Non-destructive transcriptomics via vesicular export.

Nature communications·2026
Same author

Improved survival with fludarabine-based therapies in mixed phenotype acute leukaemia: A population-based study using the WHO 2022 classification.

British journal of haematology·2026

相关实验视频

Updated: May 6, 2026

Profiling Individual Human Embryonic Stem Cells by Quantitative RT-PCR
09:03

Profiling Individual Human Embryonic Stem Cells by Quantitative RT-PCR

Published on: May 29, 2014

11.6K

使用单细胞蛋白组学和转录组学绘制早期人类血细胞分化

Benjamin Furtwängler1,2,3, Nil Üresin1,2,3, Sabrina Richter4,5

  • 1The Finsen Laboratory, Rigshospitalet, Copenhagen University Hospital, Faculty of Health Sciences, University of Copenhagen, Copenhagen, Denmark.

Science (New York, N.Y.)
|August 21, 2025
PubMed
概括
此摘要是机器生成的。

单细胞蛋白质组学 (scp-MS) 与单细胞转录组学 (scRNA-seq) 结合,揭示了单独的mRNA分析遗漏的关键蛋白质. 这种多组学方法增强了对细胞分化和功能的理解.

更多相关视频

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations
09:34

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Published on: October 25, 2018

6.8K
Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues
10:12

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Published on: January 10, 2019

18.7K

相关实验视频

Last Updated: May 6, 2026

Profiling Individual Human Embryonic Stem Cells by Quantitative RT-PCR
09:03

Profiling Individual Human Embryonic Stem Cells by Quantitative RT-PCR

Published on: May 29, 2014

11.6K
A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations
09:34

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Published on: October 25, 2018

6.8K
Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues
10:12

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Published on: January 10, 2019

18.7K

科学领域:

  • 生物化学
  • 分子生物学
  • 基因组学

背景情况:

  • 单细胞转录组学 (scRNA-seq) 广泛用于细胞异质性和分化研究.
  • 单靠mRNA测量就有可能错过关键的生物见解.
  • 单细胞水平的蛋白质组数据提供了补充信息.

研究的目的:

  • 通过质谱法 (scp-MS) 与scRNA-seq整合单细胞蛋白质组学.
  • 从mRNA数据中识别出干细胞功能关键的蛋白质.
  • 开发一个单细胞多组分析的框架.

主要方法:

  • 从超过2500个人类CD34+造血干细胞和前代细胞生成了scp-MS数据集.
  • 集成scp-MS数据与现有的scRNA-seq数据.
  • 模拟转化动态以推断细胞的进展.

主要成果:

  • 识别了干细胞功能必不可少的mRNA转录不显示的蛋白质.
  • 证明了翻译动态的建模解释了mRNA中的更多蛋白质变异而不是线性相关性.
  • 展示了整合蛋白质组和转录组数据的实用性.

结论:

  • 单细胞蛋白质组提供了超出mRNA水平的关键信息.
  • 综合的多组学方法提供了更全面的细胞过程.
  • 这项研究为先进的单细胞多组学研究奠定了基础.