平衡在保存精子功能和代谢活动中的作用在猪精液液体储存期间
Ferran Garriga1, Jodd Codina-Benaiges1, Marc Yeste2
1Biotechnology of Animal and Human Reproduction (TechnoSperm), Institute of Food and Agricultural Technology, University of Girona, ES-17003, Girona, Spain; Unit of Cell Biology, Department of Biology, Faculty of Sciences, University of Girona, ES-17003, Girona, Spain.
Theriogenology
|August 21, 2025
概括
保持 (Ca2+) 平衡对于在液体储存期间保持野猪精子质量至关重要. 细胞外Ca2+化改善了生命力和运动性,这表明一种增强精液保存的方法.
科学领域:
- 生殖生物学 生殖生物学
- 精子生理学 精子生理学
- 动物科学动物科学
背景情况:
- 离子 (Ca2+) 对于精子功能,如电容和受精至关重要.
- 在液体储存期间,Ca2+在精子保存中的作用仍未得到充分研究.
研究的目的:
- 为了研究改变细胞外和细胞内Ca2+可用性的影响猪精子质量在17°C的液体保存过程中.
- 为了确定最佳的Ca2+动态,以保持精子存储期间的生命力和功能.
主要方法:
- 在四个条件下,猪精液被储存在贝尔茨维尔解液 (BTS) 中:控制,Ca2+补充,细胞外Ca2+化 (EGTA) 和细胞内Ca2+化 (EGTA-AM).
- 使用流细胞计,氧气消耗率 (OCR) 和计算机辅助精子分析 (CASA) 来评估精子质量.
主要成果:
- 补充Ca2+和细胞内Ca2+化对精子运动,活力和代谢活动产生了负面影响.
- 细胞外Ca2+化维持了精子的生命力,膜完整性和运动性,氧气消耗率和活性氧物种的产生仅略有降低.
- 补充Ca2+诱导过早的电容性变化,损害了精子功能.
结论:
- 保持细胞外Ca2+水平对于在液体储存期间保持猪精子质量至关重要.
- 微调Ca2+稳态,而不是完全耗尽,提供了一个有前途的策略,以提高液体储存的野猪精液的寿命和受精能力.
相关概念视频
Feedback Regulation of Calcium Concentration
3.5K
Calcium is an essential signaling molecule required for various cellular functions. Calcium pumps and ion channels on cell and organellar membranes, such as those on the endoplasmic reticulum (ER), regulate calcium concentrations inside the cell. They remain closed, keeping the cytosolic calcium levels low at a resting state.
Various transmembrane receptors, such as G protein-coupled receptors (GPCRs), elicit a response to extracellular signals by increasing cytosolic calcium. Activated GPCRs...
Various transmembrane receptors, such as G protein-coupled receptors (GPCRs), elicit a response to extracellular signals by increasing cytosolic calcium. Activated GPCRs...
3.5K
Sperm Structure and Semen Composition
5.0K
During ejaculation, males release around 2-5 milliliters of semen, which is a complex mixture of mature sperm and various fluids produced by accessory glands. The mature sperm cells measure approximately 60 micrometers in length and consist of a head, neck, midpiece, and tail. The head is flattened and tapered, measuring about 4 to 5 micrometers in length. It contains a nucleus with condensed chromosomes and an acrosome, a cap-like structure filled with enzymes essential for penetrating the...
5.0K
Skeleton and Calcium Homeostasis
4.8K
Calcium is not only the most abundant mineral in bone but also the most abundant mineral in the human body. Calcium ions are needed for bone mineralization, tooth health, heart rate regulation and strength of contraction, blood coagulation, the contraction of smooth and skeletal muscle cells, and the regulation of nerve impulse conduction. The average calcium level in the blood is about 10 mg/dL. When the body cannot maintain this level, a person will experience hypo or hypercalcemia.
4.8K
Spermatogenesis
103.4K
Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male...
103.4K
Sperm Transport
1.4K
The journey of sperm from its origin to the point of ejaculation begins within the seminiferous tubules of the testis. Here, Sertoli cells produce fluid that propels non-motile sperm through a series of conduits, starting with the straight tubules leading to the rete testis. This interconnected network of tubules acts as the initial pathway for sperm, guiding them into the efferent ductules and then into the epididymis for maturation.
The maturation phase occurs in the epididymis, where sperm...
The maturation phase occurs in the epididymis, where sperm...
1.4K
Synthesis and Functions of Calcitonin
2.4K
Calcitonin, a vital polypeptide hormone, regulates calcium levels within body fluids. It is released by the parafollicular cells, also known as C cells, situated in the follicular epithelium of the thyroid gland. Calcitonin responds to fluctuations in blood calcium levels and the influence of gastrointestinal hormones like gastrin and cholecystokinin.
The exact mechanisms by which calcitonin operates in calcium homeostasis remain elusive, but its significance is evident in several vital...
The exact mechanisms by which calcitonin operates in calcium homeostasis remain elusive, but its significance is evident in several vital...
2.4K


