Search

Search

这页已由机器翻译。其他页面可能仍然显示为英文。 View in English

首页
...
研究领域生物医学和临床科学瘤学和致癌症预测和预后标志物
在HPV16+宫癌治疗后对HPV16 L2E7E6融合蛋白接种部位进行随机试验

在HPV16+宫癌治疗后对HPV16 L2E7E6融合蛋白接种部位进行随机试验

Stéphanie Gaillard ¹, Jade Alvarez ², Tianbei Zhang ³, Hao Wang ⁴, Hua-Ling Tsai ⁴, Leslie Cope ⁴, Amy Deery ⁵, Vikrant Palande ², Chi-Fen Lee ², Amanda N Fader ⁶, Warner K Huh ⁷, Rebecca C Arend ⁷, Margaret I Liang ⁷, J Michael Straughn ⁷, Russell Vang ², Darin Ostrander ⁸, Karen Horner ⁸, Li Zhang ⁹, Dipika Singh ⁹, Kellie N Smith ³, T C Wu ¹⁰, Charles A Leath ⁷, Richard B S Roden ¹⁰

Stéphanie Gaillard ¹, Jade Alvarez ², Tianbei Zhang ³

¹Department of Oncology, Johns Hopkins School of Medicine; Baltimore, MD, USA; Department of Gynecology/Obstetrics, Johns Hopkins School of Medicine; Baltimore, MD, USA.
²Department of Pathology, Johns Hopkins School of Medicine; Baltimore, MD, USA.
³Department of Oncology, Johns Hopkins School of Medicine; Baltimore, MD, USA; Bloomberg∼Kimmel Institute for Cancer Immunotherapy, Johns Hopkins School of Medicine; Baltimore, MD, USA; Mark Foundation Center for Advanced Genomics and Imaging, Johns Hopkins University, Baltimore, MD, USA.
⁴Department of Oncology, Johns Hopkins School of Medicine; Baltimore, MD, USA; Department of Biostatistics, Johns Hopkins School of Medicine; Baltimore, MD, USA.
⁵Department of Oncology, Johns Hopkins School of Medicine; Baltimore, MD, USA.
⁶Department of Gynecology/Obstetrics, Johns Hopkins School of Medicine; Baltimore, MD, USA.
⁷Division of Gynecologic Oncology, University of Alabama at Birmingham; Birmingham, AL, USA.
⁸Department of Nephrology, Johns Hopkins School of Medicine; Baltimore, MD, USA.
⁹Department of Oncology, Johns Hopkins School of Medicine; Baltimore, MD, USA; Bloomberg∼Kimmel Institute for Cancer Immunotherapy, Johns Hopkins School of Medicine; Baltimore, MD, USA.
¹⁰Department of Oncology, Johns Hopkins School of Medicine; Baltimore, MD, USA; Department of Gynecology/Obstetrics, Johns Hopkins School of Medicine; Baltimore, MD, USA; Department of Pathology, Johns Hopkins School of Medicine; Baltimore, MD, USA.

⁰Department of Oncology, Johns Hopkins School of Medicine; Baltimore, MD, USA; Department of Gynecology/Obstetrics, Johns Hopkins School of Medicine; Baltimore, MD, USA.

Gynecologic Oncology +

|

2025年八月21日

相关实验视频

These videos have been matched automatically. Contact us if they are not relevant.

Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus

Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus

Published on: March 8, 2012

Chromogenic In Situ Hybridization as a Tool for HPV-Related Head and Neck Cancer Diagnosis

Chromogenic In Situ Hybridization as a Tool for HPV-Related Head and Neck Cancer Diagnosis

Published on: June 14, 2019

RNAscope for <em data-lazy-src=

RNAscope for In situ Detection of Transcriptionally Active Human Papillomavirus in Head and Neck Squamous Cell Carcinoma

Published on: March 11, 2014

See all related videos

在PubMed上查看摘要

概括

此摘要是机器生成的。

与手臂疫苗接种相比,用TA- CIN (HPV16 L2E7E6融合蛋白) 接种大腿疫苗对宫癌患者产生了更强的免疫反应,包括更高的抗体和CD8 T细胞活性. 在没有疾病迹象的患者中,这种方法是安全的,并且耐受良好.

科学领域

免疫学
癌症学
疫苗学

背景情况

在临床前模型中,通过疫苗接种策略可以增强抗瘤免疫力.
人类乳头瘤病毒16型 (HPV16) 是宫癌的主要致癌因素.
HPV16 L2E7E6融合蛋白 (TA-CIN) 是一个候选治疗疫苗.

研究的目的

在HPV16+宫癌患者中,比较TA- CIN在大腿和手臂中的免疫性.
评估TA-CIN疫苗接种在初级治疗后没有疾病迹象的患者的安全性和有效性.

主要方法

一项试点研究随机选择了15名没有疾病迹象的HPV16+宫癌患者,每月在手臂或大腿接受三次肌内TA- CIN免疫接种 (100微克).
使用CTCAEv4. 0标准评估安全性,并通过抗体标位,批量T细胞受体测序 (TCRseq) 和单细胞RNA测序 (scRNAseq) 分析免疫反应.
对患者进行了两年的复发跟踪.

主要成果

TA-CIN疫苗接种耐受性良好,主要是注射部位发生1级治疗相关的不良事件.
与手臂疫苗接种相比,大腿疫苗接种导致TA- CIN特异性抗体标位更高,T细胞克隆扩张明显更大 (包括E6,E7和L2特异性).
在接种疫苗后,scRNAseq发现了MAIT和细胞毒性CD8+T细胞的显著扩张,主要是L2特异性T细胞反应和新型TCR克隆型.

结论

在没有疾病迹象的HPV16+宫癌患者中,肌肉内TA- CIN疫苗接种是安全的,并引起强烈的免疫反应.
与手臂接种疫苗相比,大腿接种疫苗表现出优异的免疫性,诱导更高的CD8T细胞和抗体反应.
作为HPV16驱动癌症的治疗策略,TA- CIN疫苗需要进一步研究.

关键词:

宫癌没有. 美国在HPV16 一个L2 免疫疗法

相关实验视频

这些视频已自动匹配 Contact us 如果它们不相关

Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus

Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus

Published on: March 8, 2012

Chromogenic In Situ Hybridization as a Tool for HPV-Related Head and Neck Cancer Diagnosis

Chromogenic In Situ Hybridization as a Tool for HPV-Related Head and Neck Cancer Diagnosis

Published on: June 14, 2019

RNAscope for <em>In situ</em> Detection of Transcriptionally Active Human Papillomavirus in Head and Neck Squamous Cell Carcinoma

RNAscope for In situ Detection of Transcriptionally Active Human Papillomavirus in Head and Neck Squamous Cell Carcinoma

Published on: March 11, 2014

See all related videos

相关概念视频

Cancer Vaccines

Cancer Vaccines

Cancer treatment vaccines are a rapidly evolving field that offers a promising approach to immunotherapy. Unlike traditional vaccines that prevent diseases, cancer treatment vaccines are designed to treat existing cancers by stimulating the immune system to recognize and attack cancer cells.
Cancer vaccines come in two categories: preventive (prophylactic) and treatment (active). Preventive vaccines, such as the Human Papillomavirus (HPV) vaccine, protect against viruses that cause certain...

Treatment Resistant Cancers

Treatment Resistant Cancers

Cancer is the second leading cause of death in the United States. A cancer cell is genetically unstable and hence can mutate faster. They can also modify their microenvironment and escape immune surveillance. The difficulties in treating cancer are further compounded by the emergence of rapid resistance to anticancer drugs. The most common ways to attain resistance in cancer cells include alteration in drug transport and metabolism, modification of drug target, elevated DNA damage response, or...

Share

X
Facebook
LinkedIn
YouTube

ABOUT JoVE

Overview
Leadership
Blog
JoVE Help Center

AUTHORS

Publishing Process
Editorial Board
Scope & Policies
Peer Review
FAQ
Submit

LIBRARIANS

Testimonials
Subscriptions
Access
Resources
Library Advisory Board
FAQ

RESEARCH

JoVE Journal
Methods Collections
JoVE Encyclopedia of Experiments
Archive

EDUCATION

JoVE Core
JoVE Business
JoVE Science Education
JoVE Lab Manual
Faculty Resource Center
Faculty Site

Terms & Conditions of Use
Privacy Policy
Policies