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使用HPLC-MS/MS对Bufei Huoxue囊中的20种化合物的药理动力学评估

Wenwen Li¹, Jinyue Ma¹, Zijing Zhang¹

¹State Key Laboratory of Chinese Medicine Modernization, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China.

Journal of pharmaceutical and biomedical analysis

|August 21, 2025

概括

这项研究开发了一种经过验证的HPLC-MS/ MS方法,用于在老鼠血中量化Bufei Huoxue囊 (BHC) 中的20种化合物,从而实现改善肺部状况的药理学分析.

科学领域:

药理学和中国传统医学分析
分析化学和质谱学

背景情况:

这是一种用于治疗肺部疾病的中国传统药物.
对BHC的活性成分及其药理动力学的全面了解对于优化其治疗用途至关重要.

研究的目的:

开发和验证一种新的高性能液体染色学三极四极质谱法 (HPLC-MS/MS).
在大鼠血中定量确定20个BHC的关键成分.
在口服后研究这些化合物的药物动力学特征.

主要方法:

使用HPLC-MS/MS同时量化大鼠血中的20种化合物.
验证了该方法的线性,精度,准确性,回收,矩阵效应和稳定性.
应用了经过验证的方法来分析用BHC治疗的老鼠的血样本.

主要成果:

通过HPLC-MS/MS方法显示出极好的线性 (r ≥ 0. 9926),精度 (RSD < 13. 41%) 和准确性 (-13. 56%至 12. 97%).
提取回收率在62.17%至96.05%之间,矩阵效应在74.42%至115.96%之间.
所有20种化合物在各种储存条件下都表现出良好的稳定性.

结论:

一种可靠且经过验证的HPLC-MS/MS方法成功地确定了20个BHC组件.
该方法为BHC的药理动力学研究提供了可靠的工具.
这些发现支持BHC在肺部疾病的进一步研究和临床应用.

关键词:

布菲黄泉囊在HPLC-MS/MS中药物动力学小鼠的血

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