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对海洋甲动物生物多样性的eDNAmetabarcoding中COI原料的有效性进行评估

Wenting Ren¹, Peng Zhou², Dongsheng Zhang³

¹State Key Laboratory of Submarine Geoscience, School of Oceanography, Shanghai Jiao Tong University, Shanghai, 200030, China; State Key Laboratory of Submarine Geoscience, Second Institute of Oceanography, Ministry of Natural Resources, Hangzhou, 310012, China; Key Laboratory of Marine Ecosystem Dynamics, Second Institute of Oceanography, Ministry of Natural Resources, Hangzhou, 310012, China.

Marine environmental research

|August 21, 2025

概括

环境DNA (eDNA) 代码使用线粒体COI基因标记物进行生物多样性评估. 这项研究评估了四组COI原料,发现对3 (mlCOIintF-XT/jgHCO2198) 对海洋元动物最有效.

科学领域:

海洋生物学
分子生态学
生物信息学

背景情况:

环境DNA (eDNA) 代码越来越多地用于生物多样性评估,特别是在具有挑战性的海洋环境中.
基质特异性和PCR偏差的限制可能会影响eDNA生物多样性评估的准确性.
缺乏对海洋eDNA元编码的量化评估.

研究的目的:

量化评估用于海洋eDNA元编码的四种常用线粒体COI原始组的性能.
确定海洋元动物的最佳放大效率和分类学覆盖范围.
为改善eDNA研究中的原料选择提供指导.

主要方法:

在中使用PCR来评估四组COI原料.
来自NCBIRefSeq数据库的4267个COI基因序列的分析.
对主要海洋类的原料特异性,放大效率和分类学覆盖的评估.

主要成果:

在四组初始化器中观察到放大效率和分类学覆盖的显著差异.
对于大多数海洋甲基动物来说,初级组对3 (mlCOIintF-XT/jgHCO2198) 显示出优异的性能.
扩增成功率在主要科目中从81.6%到99.4%不等,某些科目 (例如,Cnidaria,Porifera) 被低估了.

结论:

选择原料对海洋eDNA生物多样性评估的准确性和全面性产生重大影响.
推使用mlCOIintF-XT/jgHCO2198初始化套件进行广泛的海洋甲基动物eDNA甲基编码.
标准化原料选择可以提高电子DNA研究的可比性和可重复性.

关键词:

生物多样性一个 COI 基因原料海洋甲基动物不匹配偏差电子DNA元编码

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