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通过上调表达和活性增强,通过铜对阿佐尔降解酶SaCinD的双重激活

Jingwen Fan¹, Tuoyu Zhou², Aman Khan³

¹Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, Lanzhou, Gansu 730000, China; Centre for Grassland Microbiome, State Key Laboratory of Herbage Improvement and Grassland Agro-ecosystems, Lanzhou University, Lanzhou, Gansu 730000, China.

Journal of hazardous materials

|August 21, 2025

概括

这项研究显示,铜 (II) 增强了黄杆菌LZ-01中的亚索降解酶活性和表达,改善了染料排毒. 这种铜介导的双重激活机制为废水处理提供了有前途的生物修复策略.

科学领域:

环境微生物学
生物技术
生物修复

背景情况:

已知铜 (Cu) 能加速微生物染料的解毒.
不完全了解Cu(II) 影响亚还原酶表达和功能的具体机制.
在废水中染料的降解中,降解细菌起着至关重要的作用.

研究的目的:

研究Cu (II) 在LZ-01黄金葡萄球菌中阿佐雷杜克酶的表达和活性中的作用.
为了阐明Cu (II) 中介染料排毒的分子机制.
评估该系统对染料污染废水的生物修复潜力.

主要方法:

在金色葡萄球菌LZ-01中识别和表征Cu (II) 依赖的亚缩酶基因 (SaCinD).
使用流细胞计和光显微镜分析铜调节器SaCopR的基因调节.
酶动力学 (K_m,V_max) 和异热定位热量测量 (ITC) 来确定Cu(II) 结合和对SaCinD活性的影响.
位点定向的突变发生,以确定SaCinD中的Cu (II) 结合位点.

主要成果:

鉴定了一种依赖Cu (II) 的阿佐雷达酶基因SaCinD,发现其表达受到SaCopR的调节.
显著增强了SaCinD活性 (V_max增加了1.5倍) 并直接与酶结合.
突变基因鉴定出Lys94和Leu156是潜在的Cu(II) 结合点,其中特定突变基因的活性和染料代谢发生了变化.
降解产品的毒性降低,SaCinD在模拟废水中表现出异常的变色能力.

结论:

提出了一种新的Cu (II) 介导的微生物解毒双重激活机制,包括增强的基因表达和酶活性.
这种机制突显了铜在促进色素降解酶功能的协同作用.
这些发现为开发含有色剂和重金属的工业废水有效的生物修复策略提供了有希望的途径.

关键词:

亚降解酶催化活动 (二) 其他染料的降解位点定向的突变发生转录表达方式

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