解开中温Daqu内部和外部层之间的Qu-aroma变化:多体和感官方法
Sheng-Bing Yang1, Zhen-Ming Lu2, Han-Jun Shen1
1Key Laboratory of Industrial Biotechnology of Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, PR China; National Engineering Research Center of Cereal Fermentation and Food Biomanufacturing, Jiangnan University, Wuxi 214122, PR China.
International journal of food microbiology
|August 21, 2025
概括
这项研究揭示了中温Daqu启动器的内部和外部层的独特香味特征和微生物群落. 了解这些差异是控制发酵产品中复杂的Qu-aroma的关键.
科学领域:
- 食品科学与技术
- 微生物学
- 生物技术
背景情况:
- 固态发酵导致微环境异质性,影响微生物群落和代谢物产生.
- 对于发酵饮料来说至关重要的达库起泡剂具有影响其芳香特征的空间变化.
研究的目的:
- 调查中温达克 (MT-Daqu) 的内外层之间的Qu-aroma变化.
- 分析与芳香挥发性有机化合物 (VOC) 相关的微生物群落差异和相关的代谢途径.
主要方法:
- 定量描述性分析和头部固态微提取气体染色体质谱 (HS-SPME-GC-MS) 用于芳香分析.
- 用于气味识别的薄膜 (TF) -SPME-GC-O/MS.
- 用于微生物群体分析的元基因组和安普利康序列.
- 与代谢数据库进行交叉引用以将VOC与代谢途径联系起来.
主要成果:
- 独特的香味特征:内层 (生烤),外层 (颗粒,木质).
- 确定了225种VOC,其中43种VOC在不同层之间存在差异.
- 37种芳香活性有机化合物与6种微生物代谢模块相关联.
- 在内层 (Kroppenstedtia,Thermomyces) 和外层 (Lactobacillus,Saccharomycopsis) 中表现出不同的微生物属,其中一些属与代谢模块无处不在.
结论:
- 在MT-Daqu的空间异质性显著影响了Qu-aroma的组成.
- 特定的微生物群落及其代谢途径是层特异性芳香特征的驱动因素.
- 这项研究提供了关于Qu-aroma及其微生物基础的空间异质性的见解.
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