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植物遗传学分析,组织表达和TRP基因对温度变化的反应 Monopterus albus

Zhi Yang¹, Yuhua Sun²

¹Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China; College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing 100049, China.

Comparative biochemistry and physiology. Part D, Genomics & proteomics

|August 21, 2025

概括

在Monopterus albus中,短暂受体潜力 (TRP) 基因表现出性别特异和组织特异的表达,大多数对温度变化做出反应. Trpc1与高温和生殖组织有关,这表明它在性别决定中起作用.

科学领域:

基因组学
鱼类生物学
感官生理学

背景情况:

暂时受体潜能 (TRP) 道对于脊椎动物的感官知觉至关重要,它们对温度等环境刺激做出反应.
一项先前的研究表明,Trpv4在雌雄性鱼 (Monopterus albus) 中因温度而发生性逆转.
了解TRP基因功能对于水产养殖和适应性研究至关重要.

研究的目的:

为了表征Monopterus albus中的TRP基因家族.
研究不同性别,组织和温度的TRP基因表达模式.
探索TRP基因在温度诱导的性别逆转和环境适应中的作用.

主要方法:

从Monopterus albus获得了36个TRP基因,并对6个子家族进行了鉴定.
对来自10个物种的336个TRP基因进行了比较基因组分析.
在不同条件下 (性别,组织,温度) 使用RT-qPCR分析TRP基因表达.

主要成果:

在M. albus中确定了36个TRP基因,属于TRPA,TRPC,TRPM,TRPV,MCOLN和PKD亚家族.
对10个物种进行的比较分析显示,TRP的子家族保存,其中一些物种存在TRPN.
在M. albus中,TRP基因表达呈现出性别和组织特异性的显著差异,男性和性腺/心脏的表达高.
对TRP基因观察到四种不同的温度反应模式;大多数对高温和低温都很敏感.
仅对高温敏感的Trpc1,局限于阴道生殖细胞,并处于积极选择状态.

结论:

在Monopterus albus中,TRP基因家族显示了与性别,组织和温度相关的复杂表达模式.
特定的TRP基因,如Trpc1,可能在温度诱导的性别逆转和适应环境变化中发挥作用.
这项研究为进一步研究鱼类繁殖和环境反应中的TRP基因功能提供了基础数据.

关键词:

类动物 TRP 的基因温度变化组织表达

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