MarK,一种Novosphingobium aromaticivorans基因酶,需要用于多种芳香单体的代谢
Benjamin W Hall1, Jeanette C Neri1, Craig A Bingman2
1Department of Energy Great Lakes Bioenergy Research Center, Wisconsin Energy Institute, University of Wisconsin-Madison, Wisconsin, USA.
The Journal of biological chemistry
|August 21, 2025
概括
研究人员设计了Novosphingobium aromaticivorans来代谢乙尼龙,一种由素衍生的芳香化合物. 在Saro_1862 (MarK) 中的一种氨基酸突变通过增加酸化率使阿塞托瓦尼隆的生长成为可能.
科学领域:
- 微生物代谢和酶工程
- 生物技术和可再生资源
背景情况:
- 芳香化合物对工业至关重要,但主要来自石油.
- 这种可再生植物聚合物是芳香化合物的丰富来源.
- 诺沃辛戈 (Novosphingobium aromaticivorans) 可以代谢一些素衍生物,但不能代谢阿塞托瓦尼隆.
研究的目的:
- 为了使 Novosphingobium aromaticivorans 能够利用阿塞托瓦尼隆作为唯一的碳来源.
- 在N. aromaticivorans中确定阿塞托瓦尼隆代谢的遗传基础.
- 描述负责阿塞托瓦尼隆酸化的酶.
主要方法:
- 适应性实验室进化 N. 芳香动物.
- 基因分析以确定赋予阿塞托瓦尼隆利用的突变.
- 生物化学测定和蛋白质结晶学以描述酶活性和结构.
主要成果:
- 在Saro_ 1862中,单个氨基酸替代 (E16K) 已被确定为对阿塞托瓦尼隆的生长至关重要.
- 这种MarK E16K变体的ATP依赖性化率增加了约1600倍.
- 重组MarK E16K可化多种芳香化合物,定义UPF0261域的催化活性.
结论:
- 马克酶 (Saro_1862) 是一种新型多重芳酶.
- 通过改变其ATP结合部位,E16K替代增强了MarK的活性.
- 对芳香代谢的确定的途径可能在细菌系中保持.
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