在Lateolabrax maculatus和Micropterus salmoides中对AAT和SAA的分子表征和功能分析
Yawen Yang1, Yushuai Xie1, Zhaosheng Sun1
1Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai, 201306, China; National Pathogen Collection Center for Aquatic Animals, Shanghai Ocean University, Shanghai 201306, China.
Fish & shellfish immunology
|August 21, 2025
概括
这项研究对鱼类的α-1抗素 (AAT) 和血清氨基酸A (SAA) 基因进行了表征,发现它们在各种组织中表达,并由免疫挑战诱导,这表明它们在鱼类免疫力中起作用.
科学领域:
- 免疫学
- 遗传学
- 水产养殖
背景情况:
- 阿尔法-1抗素 (AAT) 和血清粉样蛋白A (SAA) 是感染,炎症和组织损伤的关键调节剂.
- 了解鱼类中的这些基因对于水产养殖和疾病管理至关重要.
研究的目的:
- 从斑点海 (Lateolabrax maculatus) 和大嘴海 (Micropterus salmoides) 中克隆和描述AAT和SAA基因.
- 研究这些基因在鱼类中的表达模式和免疫反应.
主要方法:
- 从两种鱼类中克隆和测序AAT和SAA的基因.
- 在各种组织中进行定量基因表达分析.
- 涉及过度基因表达的细胞局部化研究和功能测试.
主要成果:
- 这些AAT和SAA基因 (LmAAT, MsAAT, LmSAA, MsSAA) 已成功克隆和表征.
- 这些基因表现出高序列身份和与其他远星同类的进化关系.
- 在所有测试组织中检测到基因表达,主要是在肝脏中,并且受到LPS和Edwardsiella tarda的显著诱导.
- LmAAT和MsAAT定位在血上,而LmSAA和MsSAA则定位在细胞核上.
- 在鱼脑细胞中,LmAAT或MsAAT的过度表达诱导了TNFα表达.
结论:
- 克隆的鱼类AAT和SAA基因与其他脊椎动物中的同类共享保存特征.
- 这些基因在鱼类的天生的免疫反应中起作用,对细菌的挑战做出反应.
- 独特的细胞局部化和TNFα诱导表明AAT和SAA在鱼类免疫信号传递中的特定作用.
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