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基于TRSV的实时可视化基因沉默方法,使用体作为黄瓜中的选择标记物

Xu Wang¹, Chenhao Zhou¹, Xiaoli Gao¹

¹Hainan Provincial Key Laboratory of Quality and Safety for Tropical Fruits and Vegetables, Key Laboratory of Quality and Safety Control of Subtropical Fruits and Vegetables, Ministry of Agriculture and Rural Affairs, College of Horticulture Science, Zhejiang Agriculture and Forestry University, Hangzhou, Zhejiang 311300, China.

Plant science : an international journal of experimental plant biology

|August 21, 2025

概括

在黄瓜中,一种新的病毒诱导基因沉默 (VIGS) 系统使用三角体作为视觉标记. 这种TRSV-CsGL3系统简化了基因功能研究,并允许在整个植物中实时观察

科学领域:

植物分子生物学
遗传学
农业科学

背景情况:

黄瓜 (Cucumis sativus L.) 是一个全球重要的蔬菜作物.
虽然黄瓜的基因组已经被测序,
病毒诱导的基因沉默 (VIGS) 对于基因功能分析是有效的,但传统的检测方法耗时.

研究的目的:

开发一个高效,可视检测的黄瓜VIGS系统.
建立黄瓜基因功能研究的实时VIGS方法.
用三角体的发展作为VIGS效率的视觉标记.

主要方法:

在黄瓜中开发真空农业透的基于烟草环状点病毒 (TRSV) 的VIGS系统.
将 CsGLABROUS3 (CsGL3) 克隆到 pTRSV2 载体中以创建 TRSV-CsGL3 系统.
使用CsGL3 (三胞体启动) 和CsUFO (花形发生) 基因验证该系统.

主要成果:

通过TRSV-CsGL3系统,在黄瓜中实现了可见的基因沉默,在整个植物生命周期中观察到无毛表型.
抑制CsUFO基因导致预期的花发育缺陷.
三体表型作为VIGS效率的有效实时视觉标志物.

结论:

对于黄瓜,TRSV-CsGL3系统提供了简化和实时可视化的VIGS方法.
这种系统可以在整个黄瓜生命周期中有效地研究基因功能.
使用三体作为选定标记物简化了VIGS识别程序.

关键词:

在CGL3 黄瓜实时可视化美国三体其他类型

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