用于调节植物相关的Trichoderma中的克拉米多形成的双类生物合成的非正规的类循环
Min-Jie Yang1, De-Sen Li1, Hua-Qin Deng1
1State Key Laboratory of Phytochemistry and Natural Medicines, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, P. R. China.
Nature communications
|August 21, 2025
概括
研究人员在Trichoderma真菌中发现了新型的烯循环酶 (TriDTC). 这些酶产生复杂的二基,对于真菌生存和生物控制应用至关重要.
科学领域:
- 生物化学
- 菌群学
- 自然产品化学
背景情况:
- 三甲体真菌产生复杂的二甲体 (harzianes,trichodermanins),其生物合成和功能尚不清楚.
- 基环酶是基骨形成中的关键酶.
- 了解这些途径对于生物控制和药物发现至关重要.
研究的目的:
- 识别和描述Trichoderma中的新型环.
- 阐明这些酶的机制和进化起源.
- 研究产生的二类生物的作用.
主要方法:
- 候选基因的异质表达
- 基因删除和体外酶测试.
- 遗传学分析和功能研究.
主要成果:
- 鉴定了TriDTCs,这是Trichoderma的一种新型环酶家族.
- 已证实TriDTC可循环将格拉尼尔二酸盐 (GGPP) 转化为哈尔齐安醇I和维克罗尔A.
- 发现的TriDTC具有独特的催化机制和进化历史.
- 将TriDTC与耐药性真菌细胞的形成联系在一起.
结论:
- TriDTC 是一种具有独特特征的新型环类.
- 这些酶对Trichoderma的生存策略至关重要.
- 这些发现有助于提高生物控制的有效性和探索二类药理学.
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