相关实验视频

Updated: Sep 10, 2025

Planarian Immobilization, Partial Irradiation, and Tissue Transplantation

Planarian Immobilization, Partial Irradiation, and Tissue Transplantation

Published on: August 6, 2012

从即将发生的细胞死亡中进行编程的细胞复苏可促进组织的修复和再生

Kollori Dhar^1,2,3, Kautilya Kumar Jena², Subhash Mehto⁴

¹CSIR-Centre for Cellular and Molecular Biology, Hyderabad, Telangana, 500007, India.

The EMBO journal

|August 21, 2025

概括

细胞可以通过涉及基因激活的编程过程从临死状态恢复生长和修复. 这种细胞复苏机制对于再生至关重要,

科学领域:

细胞生物学
复原医学
发育生物学

背景情况:

细胞从接近死亡状态的恢复还不太清楚.
像L-leucyl-L-leucine甲基 (LLOMe) 这样的 lysosomotropic 药物可以诱导细胞死亡,但也可以触发细胞复苏.
编程细胞复苏是维持组织平衡和生物生存的关键过程.

研究的目的:

描述暴露于细胞死亡诱导剂后的细胞复苏过程.
研究细胞复苏和再生的分子机制.
探索细胞复苏在再生医学中的治疗潜力.

主要方法:

将细胞暴露在L-leucyl-L-leucine甲基 (LLOMe) 中以诱导临死状态.
在细胞恢复过程中对基因表达和染色质可访问性的分析.
在小鼠,Drosophila melanogaster,青和Caenorhabditis elegans体内研究以评估再生能力.
对NF-κB信号通路的遗传和药理操作.

主要成果:

与发育,再生,干细胞和炎症相关的基因的可获得性和升高调节的编程细胞复苏过程.
代谢,有机体生物发生,膜运输,运输和细胞骨架重塑通路的激活导致了细胞的完全更新.
在小鼠中,LLOMe增强了皮肤伤口和角膜烧伤的愈合,在Drosophila中促进了造血干细胞的产生,在青中诱导了尾巴的再生,在C. elegans中促进了轴突的再生.
确定NF-κB信号对细胞复苏和再生都至关重要.

结论:

细胞从接近死亡的状态中复苏是一个被编程的,细胞内在的机制.
这一过程涉及到发育,再生和修复途径的协调激活.
这些发现表明细胞复苏机制可以在再生医学中用于治疗.

关键词:

亚纳斯塔西斯时间表 lysosomal 细胞死亡和复生细胞复苏和再生中的NF-B信号编程的细胞再生

更多相关视频

Generation of Induced Pluripotent Stem Cells from Human Melanoma Tumor-infiltrating Lymphocytes

Generation of Induced Pluripotent Stem Cells from Human Melanoma Tumor-infiltrating Lymphocytes

Published on: November 11, 2016

De Novo Generation of Somatic Stem Cells by YAP/TAZ

De Novo Generation of Somatic Stem Cells by YAP/TAZ

Published on: May 7, 2018

相关概念视频

Tissue Renewal without Stem Cells

Tissue Renewal without Stem Cells

After cellular or tissue damage, the resident stem cells present in the human body can locally repair and regenerate the damaged tissue or organ. However, even though some tissues do not have stem cells, they can repair and regenerate with the help of pre-existing cells. For example, beta cells of the pancreas and hepatocytes of the liver can divide to renew and regenerate the tissue. Here, both cell division and cell death are well regulated by homeostasis.
However, failure of such a system...

iPS Cell Differentiation

iPS Cell Differentiation

The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.

Somatic to iPS Cell Reprogramming

Somatic to iPS Cell Reprogramming

Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...

Overview of Regeneration and Repair

Overview of Regeneration and Repair

Regeneration and repair processes are critical in healing damages caused by injury, disease, and aging. In regeneration, the damaged tissue is entirely replaced with new growth that restores the original architecture and function. In contrast, tissue repair usually results in a fixed tissue architecture involving scar formation. Scars generally do not reestablish tissue function and may also exhibit structural abnormalities at the injury site.
Regeneration
All animals have varying degrees of...

Whole Body Regeneration

Whole Body Regeneration

Regeneration is the process of restoring injured or lost tissues, organs, or body parts. While simpler organisms generally show greater ability to regenerate their whole body, few complex animals show similarly exceptional regeneration. For example, planarian flatworms have a unique regenerative potential making them a popular study organism among biologists to understand the mechanisms of whole body regeneration. Other organisms, such as hydra, also show extreme regeneration potential;...

Stem Cell Therapy for Tissue Regeneration

Stem Cell Therapy for Tissue Regeneration

Stem cell therapy is a method used in regenerative medicine to repair and restore function to damaged tissues and organs. Stem cells have the potential to proliferate and differentiate into various tissue types, making them ideal candidates for tissue regeneration. For example, hematopoietic stem cell transplants are commonly used in blood cancer treatment to replenish damaged bone marrow and restore healthy blood cells.
Types of Stem Cells used in Stem Cell Therapy
The two main cell...