Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Production Efficiency01:01

Production Efficiency

15.7K
Net production efficiency (NPE) is the efficiency at which organisms assimilate energy into biomass for the next trophic level. Due to low metabolic rates and less energy spent on thermoregulatory processes, the NPE of ectotherms (cold-blooded animals) is 10 times higher than endotherms (warm-blooded animals).
15.7K
Free-body Diagram01:28

Free-body Diagram

4.4K
In mechanics, understanding the motion of objects is essential, and one tool that helps solve this problem is the free-body diagram. It is a simple but powerful graphical representation that succinctly represents all the forces acting on an object. A free-body diagram can represent a stationary or moving object, and is used in mechanics to explain the cause of an object's motion.
A free-body diagram transforms a complex problem into a simple representation, making it easy to understand the...
4.4K
Mechanical Efficiency of Real Machines01:14

Mechanical Efficiency of Real Machines

1.6K
The mechanical efficiency of a machine is a fundamental concept that describes how effectively a machine can convert input work into output work. According to this concept, the efficiency of a machine is equal to the ratio of the output work to the input work. An ideal machine, meaning a machine that has no energy losses, has an efficiency of one. This implies that the input work and the output work are equal.
However, in reality, no machine can be truly ideal, and all of them experience some...
1.6K
Machines: Problem Solving II01:30

Machines: Problem Solving II

791
Machines are complex structures consisting of movable, pin-connected multi-force members that work together to transmit forces. Consider a lifting tong carrying a 100 kg load. It comprises movable sections DAF and CBG linked together with member AB.
791
Composite Bodies00:55

Composite Bodies

1.6K
A composite body is a body made up of multiple parts, connected to form a larger, unified object. Each part has its own weight and center of gravity, which must be considered to determine the center of gravity of the composite body. In cases where the density or specific weight is constant, the center of gravity coincides with the centroid.
Composite bodies have widespread applications in mechanical engineering, from automobiles to aircraft to rockets. For example, an automobile wheel comprises...
1.6K
Production of Formed Elements01:34

Production of Formed Elements

7.5K
Hemangioblasts are multipotent stem cells originating from the mesoderm. They give rise to hematopoietic stem cells (HSCs), which undergo hematopoiesis to produce all the formed elements of blood. This process is regulated by a complex network of hematopoietic growth factors, including transcription factors, growth factors, and cytokines. These factors stimulate the HSCs to divide and differentiate, though some HSCs remain undifferentiated to maintain a self-renewing pool.
Most HSCs commit to...
7.5K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Recent Advances in Vision-Based Beef Cattle Body Measurement Technologies.

Animals : an open access journal from MDPI·2026
Same author

A knowledge-guided deep learning framework for quantitative nucleic acid testing.

Briefings in bioinformatics·2026
Same author

Characterization, evolutionary analysis, and expression profiling of the <i>VrPYL</i> gene family in mung bean in response to abiotic stress.

PeerJ·2026
Same author

Comparison of the efficacy of different orthotopic neobladder reconstruction techniques following radical cystectomy for bladder cancer: a network meta-analysis.

Frontiers in oncology·2026
Same author

Optical cooling by interfacial charge transfer in 2D heterostructures.

Nature·2026
Same author

A 0D/3D nanoreactor integrating quantum dots and ZIF-67 for efficient urea electrosynthesis with substrate enrichment and coupling.

Chemical communications (Cambridge, England)·2026

相关实验视频

Updated: May 2, 2026

From a Natural Product to Its Biosynthetic Gene Cluster: A Demonstration Using Polyketomycin from Streptomyces diastatochromogenes T&#252;6028
09:08

From a Natural Product to Its Biosynthetic Gene Cluster: A Demonstration Using Polyketomycin from Streptomyces diastatochromogenes Tü6028

Published on: January 13, 2017

17.3K

为了高效地生产体,工程体

Xiaoxu Lin1, Xingwang Xu1, Dongxu Zhang1

  • 1State Key Laboratory of Natural Medicines, School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing 211198, China.

Journal of agricultural and food chemistry
|August 22, 2025
PubMed
概括

链杆菌宿主被设计为可扩展的化物生产,克服了传统宿主的局限性. 这项研究通过优化代谢途径实现了包括氧化衍生物在内的四种复杂类的高产量.

关键词:
杆菌的底盘工程甲基酸路径过度生产化物

更多相关视频

A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products
07:59

A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products

Published on: October 4, 2019

9.9K
A High-Yield Streptomyces Transcription-Translation Toolkit for Synthetic Biology and Natural Product Applications
07:59

A High-Yield Streptomyces Transcription-Translation Toolkit for Synthetic Biology and Natural Product Applications

Published on: September 10, 2021

4.2K

相关实验视频

Last Updated: May 2, 2026

From a Natural Product to Its Biosynthetic Gene Cluster: A Demonstration Using Polyketomycin from Streptomyces diastatochromogenes T&#252;6028
09:08

From a Natural Product to Its Biosynthetic Gene Cluster: A Demonstration Using Polyketomycin from Streptomyces diastatochromogenes Tü6028

Published on: January 13, 2017

17.3K
A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products
07:59

A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products

Published on: October 4, 2019

9.9K
A High-Yield Streptomyces Transcription-Translation Toolkit for Synthetic Biology and Natural Product Applications
07:59

A High-Yield Streptomyces Transcription-Translation Toolkit for Synthetic Biology and Natural Product Applications

Published on: September 10, 2021

4.2K

科学领域:

  • 代谢工程
  • 合成生物学
  • 自然产品生物合成

背景情况:

  • 类植物具有重要的农业和治疗潜力,但由于自然丰度低和复杂的氧化定制,它们面临生产挑战.
  • 传统的微生物宿主往往缺乏必要的酶机制,特别是细胞P450,以有效地产生复杂的衍生物.

研究的目的:

  • 为可扩展的结构复杂的化物生产开发替代微生物底盘.
  • 利用Streptomyces的生物合成能力来增强类合成和氧化定制.

主要方法:

  • 设计了两个Streptomyces宿主作为替代生产底盘.
  • 采用了EcoMine策略来识别和组装含有MEP通路基因的TriMEP磁带.
  • 优化培养基和利用强大的基因表达促进剂.

主要成果:

  • 在震动瓶培养中实现了四种特类的高水平生产,其中包括三种氧化产品.
  • 报告的产量包括 (-) -epi-α-bisabolol (237 ± 14 mg/L),ent-(13Z) -isocupressic 酸 (173 ± 14 mg/L),ent-(13E) -isocupressic 酸 (209 ± 20 mg/L) 和ent-atiserenoic 酸 (325 ± 17 mg/L).
  • 在工程化Streptomyces宿主中证明了成功的P450氧化调整.

结论:

  • 链杆菌作为一个坚固且未被充分利用的微生物底盘,用于可持续生产复杂的类.
  • 开发的代谢工程策略可以获得有价值的氧化衍生物.