相关实验视频
Updated: Sep 10, 2025

07:43
Assaying Proteasomal Degradation in a Cell-free System in Plants
Published on: March 26, 2014
14.7K
逆流体通过大米爆发真菌中的明显真空蛋白酶调节宏观和微观自
Dingyang Zhang1, Jiexiong Hu1, Yonghe Hong1,2
1State Key Laboratory of Agricultural and Forestry Biosecurity & Key Lab of Biopesticide and Chemical Biology, Ministry of Education, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
|August 22, 2025
概括
逆转基因复合体对于Magnaporthe oryzae的自是必不可少的,确保真空蛋白酶被用于载荷降解. 这种规定会影响真菌的发育和病原性.
科学领域:
- 分子生物学
- 细胞生物学
- 菌群学
背景情况:
- 这种真空体通过内细胞和自来降解细胞载荷.
- 逆转基因复合物调解从内分体中提取载荷,防止过早的降解.
- 在自过程中调节真空蛋白酶的作用尚不清楚.
研究的目的:
- 研究逆转子复合体在Magnaporthe oryzae的自过程中调节真空蛋白质溶解系统的功能.
- 确定逆转子是否影响真空蛋白酶的传递和活性,这对于自基质降解至关重要.
主要方法:
- 在M. oryzae中利用了逆转基因组件 (例如MoVps35) 和真空蛋白酶 (MoPrb1,MoPep4) 的遗传删除突变.
- 使用光显微镜来追踪真空蛋白酶的局部化 (例如,MoPrb1-GFP).
- 通过MoPrb1的催化残留的突变评估自流,并观察到真菌的发展和致病性.
主要成果:
- 包括MoVps35在内的复原体组件是真空蛋白酶 (MoPrb1) 传递到真空光的必要条件.
- 删除逆转基因组件或MoPrb1会损害自流,结合和植物感染.
- MoVps35与MoPep4相互作用,而MoPep4的丧失会影响微自 (pexophagy).
结论:
- 在M. oryzae中,逆转酶复合体在调节宏观和微观自方面起着至关重要的作用.
- 逆流体确保提供必要的真空蛋白酶,如MoPrb1和MoPep4,以有效降解载荷.
- 这种途径对真菌发育,致病性和整体细胞平衡至关重要.
更多相关视频
相关概念视频
Autophagy
4.6K
Autophagy is a self-digesting process by which a cell protects itself from threats both within and outside the cell, ranging from abnormal proteins to invading bacteria. In this process, obsolete components of the cell and invading microbes are degraded by hydrolytic enzymes active in an acidic environment of the lysosomal lumen.
An autophagic pathway consists of a series of signaling events activated in response to diverse stress and physiological conditions such as food deprivation,...
An autophagic pathway consists of a series of signaling events activated in response to diverse stress and physiological conditions such as food deprivation,...
4.6K
Export of Misfolded Proteins out of the ER
3.9K
After folding, the ER assesses the quality of secretory and membrane proteins. The correctly folded proteins are cleared by the calnexin cycle for transport to their final destination, while misfolded proteins are held back in the ER lumen. The ER chaperones attempt to unfold and refold the misfolded proteins but sometimes fail to achieve the correct native conformation. Such terminally misfolded proteins are then exported to the cytosol by ER-associated degradation or ERAD pathway for...
3.9K
Delivery Pathways to the Lysosome
7.1K
Eukaryotic cells use different mechanisms to eliminate toxic waste obsolete and worn-out substances. Lysosomes play a pivotal role in this, and hence, these substances are carried to the lysosome from other parts of the cell and extracellular space through different pathways. The most elaborately studied pathways to the lysosome are the endocytic pathways.
Endocytosis
In endocytosis, the cell membrane takes up macromolecules and particles from the surrounding medium. Clathrin-mediated...
Endocytosis
In endocytosis, the cell membrane takes up macromolecules and particles from the surrounding medium. Clathrin-mediated...
7.1K
Intralumenal Vesicles and Multivesicular Bodies
3.7K
Intraluminal vesicles (ILVs) are small vesicles 50-80 nm in diameter formed during the maturation of early endosomes. A specialized endosome containing numerous ILVs is called a multivesicular body (MVB). ILVs contain internalized molecules such as antigens, nucleic acids, proteins, and metabolites. Some of these molecules are released from the MVBs inside exosomes and are transported to other cells. Other MVBs contain molecules that are retained in the ILVs and are later degraded within the...
3.7K
Recycling Endosomes and Transcytosis
2.8K
The recycling endosome, also known as the endosomal recycling compartment (ERC), is a part of the slow-recycling process of the endocytic pathway. Molecules internalized through receptor-mediated endocytosis are either degraded in the lysosomes or are recycled to the plasma membrane through the fast- or slow-recycling route.
The recycling endosome is not a single organelle but an extensively tubulated network of recycling pathways. It functions in storing molecules or transporting them across...
The recycling endosome is not a single organelle but an extensively tubulated network of recycling pathways. It functions in storing molecules or transporting them across...
2.8K
Autophagic Cell Death
3.6K
Christian de Duve discovered “autophagy,” a process in which cellular components are engulfed by membrane-bound organelles called autophagosomes. The autophagosomes then fuse with lysosomes to digest the enclosed contents. Autophagy is generally activated in cells to prevent cell death. However, cell death is triggered when the damage is beyond repair.
Autophagy and Apoptosis
Autophagy can activate apoptosis. In normal conditions, the autophagy activating protein Beclin-1 and...
Autophagy and Apoptosis
Autophagy can activate apoptosis. In normal conditions, the autophagy activating protein Beclin-1 and...
3.6K

