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定制一个双响应部位以为基础的比度发光探头 (Ratiometric Luminescent Probe) ......II) 复杂索基架,用于监测细胞内pH值变化

Junyu Ren¹, Wenzhu Zhang¹, Xiaona Gao¹

¹School of Chemistry, Dalian University of Technology, Dalian 116024, China.

Analytical chemistry

|August 22, 2025

概括

一个新的双位点发光探头Ru-HBT准确地绘制了有机体的pH动态. 它克服了比度成像方面的挑战,提高了疾病研究的灵敏度.

科学领域:

生物医学工程生物医学工程
化学生物学化学生物学
细胞生物学细胞生物学

背景情况:

特定于有机体的pH值对于细胞平衡和疾病至关重要.
电比度发光成像提供精确的pH映射,但面临诸如光谱重叠和有限灵敏度等挑战.
现有的探测器显示在生理器官的pH范围 (4.0-8.0) 内的比率变化最小.

研究的目的:

开发一种新型的双位点比度发光探针,用于增强器官的pH传感.
克服现有的比度探测器的局限性,包括光谱重叠和灵敏度.
为了验证探头在监测细胞模型中的病态pH值变化的有效性.

主要方法:

设计和合成一个双部位的比度发光探头 (Ru-HBT) 通过合一个Ru(II) -polypyridyl复合物与一个pH敏感的西衍生物.
使用双重识别机制:光诱导电子转移 (PET) 和激发状态分子内质子转移 (ESIPT).
探测器性能的表征,包括辐射转移,发光寿命变化和辐射强度比率变化,在pH范围为4.0-9.0.0之间.

主要成果:

在单波长激发 (λex = 405 nm) 下,Ru-HBT探测器表现出很大的发射转移 (~130 nm) 和发光寿命变化 (Δτ = 226 ns).
结合PET和ESIPT机制,在器官相关的pH范围内的排放强度比率发生了354倍的显著变化.
该探测器在监测细胞亡和细胞死亡的细胞模型中的pH动态方面表现出高灵敏度和准确性.

结论:

Ru-HBT探头代表了用于器官pH监测的比度发光成像的重大进步.
它的双站点设计和集成识别机制克服了以前探测器的关键局限性,提高了检测灵敏度.
鲁-HBT是研究疾病中的病理性pH失调的宝贵工具,特别是在监测溶酶体膜透性时.

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