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Updated: Sep 10, 2025

High-resolution Spatiotemporal Analysis of Receptor Dynamics by Single-molecule Fluorescence Microscopy
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High-resolution Spatiotemporal Analysis of Receptor Dynamics by Single-molecule Fluorescence Microscopy

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单分子光数据和步数计数的贝叶斯分析和高效算法

Chiara Mattamira1, Alyssa Ward2, Sriram Tiruvadi Krishnan2

  • 1Department of Mathematics, University of Tennessee, Knoxville, TN.

Biophysical journal
|August 22, 2025
PubMed
概括

我们开发了一种新的贝叶斯方法来分析单分子光数据, 这种方法可以准确地分析复杂的生物系统,改善光数据分析.

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Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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