在口腔状细胞癌和口腔平平瘤中的Mir-let-7a差异表达:用于早期诊断生物标志物的洞察
Seyedmovahed Seyedhoseini1, Nooshin Mohtasham2, Shadi Saghafi3
1Dental Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
Reports of biochemistry & molecular biology
|August 22, 2025
概括
在口腔平瘤 (OLP) 和口腔状细胞癌 (OSCC) 患者中减少miR- let-7a表达表明其作为早期诊断生物标志物的潜力. 在OSCC中较低水平与晚期疾病相关.
科学领域:
- 生物标志物
- 分子诊断
- 癌症学
背景情况:
- 口腔平 (OLP) 是一种前恶性疾病,有可能发展为口腔状细胞癌 (OSCC).
- 微RNA,特别是miR-let-7a,正在成为关键的调节剂和潜在的诊断标记物.
- 早期发现OSCC对于有效治疗和改善患者的结果至关重要.
研究的目的:
- 评估和比较OSCC,OLP和健康对照患者的miR- let-7a表达水平.
- 确定miR-let-7a作为口腔癌及其前体病变的早期诊断标记物的潜力.
- 研究miR-let-7a表达与OSCC临床病理特征之间的相关性.
主要方法:
- 一项涉及36名OSCC患者,38名OLP患者和38名健康对照者的血清样本采集的横截面研究.
- 通过活检确认OSCC和OLP的诊断.
- 定量实时PCR (qRT-PCR) 用于测量血清中的miR-let-7a表达水平.
主要成果:
- 与健康对照组 (7. 02 ± 4. 10) 相比,OSCC (1. 55 ± 1. 19) 和OLP (2. 97 ± 2. 00) 组的miR- let-7a表达显著降低 (p< 0. 001).
- 在OSCC下降的miR- let-7a水平与较高的瘤级别 (p<0. 001),晚期 (p=0. 011),较大的瘤大小 (p<0.0001) 和淋巴结转移 (p<0.0001) 有显著的相关性.
结论:
- 在OSCC和OLP中,miR-let-7a的表达显著下降.
- 这种下调表明miR-let-7a作为一种有价值的生物标志物,可用于早期检测口腔病变的恶性转变.
- 这些发现支持miR- let-7a在OSCC和OLP疾病进展方面的作用.
相关概念视频
02:07Tumor Progression
Tumor progression is a phenomenon where the pre-formed tumor acquires successive mutations to become clinically more aggressive and malignant. In the 1950s, Foulds first described the stepwise progression of cancer cells through successive stages.
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
02:07Tumor Progression
Tumor progression is a phenomenon where the pre-formed tumor acquires successive mutations to become clinically more aggressive and malignant. In the 1950s, Foulds first described the stepwise progression of cancer cells through successive stages.
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...