Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Immunogold Electron Microscopy01:20

Immunogold Electron Microscopy

4.3K
Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.
4.3K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Femtogram-level VEGF detection via PEG-directed gold nanostructured electrochemical immunosensor.

Scientific reports·2025
Same author

Bioprocess Engineering Strategies for the Overproduction of Surface-Expressed Protein in Escherichia coli: A Review.

Biotechnology and applied biochemistry·2025
Same author

Controlled electrochemical fabrication of large and stable gold nanorods with reduced cytotoxicity.

Scientific reports·2025
Same author

Precision in cancer diagnostics: ultra-sensitive detection of MCF-7 breast cancer cells by gold nanostructure-enhanced electrochemical biosensing.

Journal of materials chemistry. B·2024
Same author

Gold nanostructure-enhanced immunosensing: ultra-sensitive detection of VEGF tumor marker for early disease diagnosis.

Scientific reports·2024
Same author

Bioluminescence measurement of superoxide anion in infertile men with oxidative stress.

Journal of photochemistry and photobiology. B, Biology·2024

相关实验视频

Updated: Sep 10, 2025

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets
06:12

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

Published on: March 17, 2023

1.6K

纳米结构的黄金平台用于attogram-precision cardiolipin量化

Mehrsa Khalilipour1, Ahmad Moshaii1,2, Hossein Siampour1

  • 1Department of Physics, Tarbiat Modares University, P.O Box 14115-175, Tehran, Iran. moshaii@modares.ac.ir.

Journal of materials chemistry. B
|August 22, 2025
PubMed
概括

这项研究引入了一种新的电化学免疫传感器,用于检测心血管疾病的生物标志物心血管蛋白. 传感器使用独特的金纳米结构进行高度敏感和选择性检测,

更多相关视频

Using Extraordinary Optical Transmission to Quantify Cardiac Biomarkers in Human Serum
09:23

Using Extraordinary Optical Transmission to Quantify Cardiac Biomarkers in Human Serum

Published on: December 13, 2017

6.4K
Single Liposome Measurements for the Study of Proton-Pumping Membrane Enzymes Using Electrochemistry and Fluorescent Microscopy
12:15

Single Liposome Measurements for the Study of Proton-Pumping Membrane Enzymes Using Electrochemistry and Fluorescent Microscopy

Published on: February 21, 2019

7.5K

相关实验视频

Last Updated: Sep 10, 2025

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets
06:12

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

Published on: March 17, 2023

1.6K
Using Extraordinary Optical Transmission to Quantify Cardiac Biomarkers in Human Serum
09:23

Using Extraordinary Optical Transmission to Quantify Cardiac Biomarkers in Human Serum

Published on: December 13, 2017

6.4K
Single Liposome Measurements for the Study of Proton-Pumping Membrane Enzymes Using Electrochemistry and Fluorescent Microscopy
12:15

Single Liposome Measurements for the Study of Proton-Pumping Membrane Enzymes Using Electrochemistry and Fluorescent Microscopy

Published on: February 21, 2019

7.5K

科学领域:

  • 电化学
  • 纳米技术
  • 生物标志物检测

背景情况:

  • 心脏脂素是线粒体功能障碍和心血管疾病的关键生物标志物.
  • 准确量化心血管蛋白对于诊断和监测心血管病理至关重要.
  • 现有的检测方法可能缺乏早期诊断所需的敏感性和选择性.

研究的目的:

  • 开发一种超灵敏的电化学免疫传感器,用于精确量化心血管蛋白.
  • 使用双金纳米结构 (纳米棒和纳米) 提高传感性能.
  • 为金纳米结构建立可复制和高效的无模板合成方法.

主要方法:

  • 通过无模板电化学沉积在氧化 (FTO) 基板上制造金纳米棒和纳米.
  • 使用场辐射扫描电子显微镜 (FESEM),X射线衍射 (XRD) 和接触角测量进行表征.
  • 电化学分析 (循环电压测量,电化学阻抗光谱) 和用抗心脂蛋白抗体进行功能化.

主要成果:

  • 实现了1 ag mL−1至0. 1 pg mL−1的超敏感心脏蛋白检测.
  • 获得的低检测极限为0.19 ag mL−1 (纳米棒) 和0.51 ag mL−1 (纳米).
  • 纳米棒具有卓越的电化学性能,包括增强的电荷传输和降低的界面电阻.

结论:

  • 开发的电化学免疫传感器提供了一个高度灵敏和选择性的心血管蛋白检测平台.
  • 金纳米结构的无模板合成为生物传感器制造提供了可扩展和可重复的方法.
  • 这项技术在脂质分析和精确医学诊断方面具有重大潜力.