使用合成化内部标准改善溶解有机物中的电子喷射电离反应线性和量化
Alexander J Craig1,2, Mustapha A Ganiyu1,3, Lindon W K Moodie2
1Analytical Chemistry, Department of Chemistry BMC, Uppsala University, Uppsala 75 237, Sweden.
Analytical chemistry
|August 22, 2025
概括
通过对电离抑制进行校正,新型化化合物提高了水中溶解有机物 (DOM) 的量化. 这种进步提高了对DOM的理解.
科学领域:
- 环境化学
- 分析化学
- 质谱学
背景情况:
- 水中溶解有机物 (DOM) 是一个复杂的混合物,使其结构的阐明和量化具有挑战性.
- 电子喷射电离高分辨率质谱 (ESI-HRMS) 已经推进了DOM分析,但由于缺乏响应因子校正,精确的量化是有限的.
研究的目的:
- 通过引入新的化化合物作为内部标准来解决准确的DOM度测定的局限性.
- 通过对电离抑制和仪器漂移进行校正来改进DOM的"伪定量化".
主要方法:
- 引入新的化化合物作为模仿DOM结构的内部标准.
- 使用d5标记的化合物对水生样本中电离抑制的评估.
- 应用化标准以改善基于度的线性和估计DOM中的酸含量.
主要成果:
- 通过对电离抑制和仪器漂移进行校正,使化碳酸丰富的标准能够进行伪定量化.
- 在使用Orbitrap的河流,沿海和深海样本中估计DOM酸含量为20-30%,使用15T FT-ICR-MS则为11-24%.
- 使用LC-MS在海水中确定了DOM化合物的~1 ng L-1特征检测极限.
结论:
- 减肥标准提供了一种增强DOM伪量化的实用方法.
- 在环境研究中可以更好地了解DOM的化学成分.
- 使用不同的质谱系统进行DOM分析的平台特定差异.
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