基于指纹残留中的氨基酸的性别分析:稳定性研究
Lu-Chuan Tian1, Ya-Bin Zhao1, Shi-Si Tian1
1People's Public Security University of China, Beijing, 100038, People's Republic of China. 20052172@ppsuc.edu.cn.
Analytical methods : advancing methods and applications
|August 22, 2025
概括
现在法医科学可以从指纹氨基酸水平来确定性别. 这种新的方法使用UPLC-Qq-MS/MS和机器学习进行准确,快速的嫌疑人追踪和人口分析.
科学领域:
- 法医化学
- 分析化学
- 生物化学
背景情况:
- 指纹中的化学残留物有助于追踪嫌疑人, 但受到生物因素的影响, 阻碍了标准化分析.
- 由于复杂的代谢来源和指纹化学成分的变化,现有的方法面临挑战.
- 司法机构需要可靠的标准化分析程序进行法医调查.
研究的目的:
- 利用指纹中的氨基酸水平,开发一种快速,准确和简单的性别预测分析方法.
- 评估相对氨基酸度作为性别确定指标的可靠性.
- 评估身体活动,生活区域和衰老对性别分类准确性的影响.
主要方法:
- 使用超高性能液体色谱-并联质谱 (UPLC-QqQ-MS/MS) 来量化指纹残留中的氨基酸.
- 机器学习技术和粒子群优化 (PSO) 为性别预测模型开发和超参数优化进行了整合.
- 进行了统计分析,包括曼- 惠特尼测试和邦费罗尼校正,以确定性别之间的显著氨基酸差异.
主要成果:
- 在特定氨基酸的相对度上观察到显著的基于性别的差异,包括Phe,Ile,Leu,Val,Pro,Asn,Glu,His和Asp.
- 在各种体力活动水平上,氨酸和氨酸呈现一致的性别差异.
- 氨酸和氨酸仍然是不同地区性别的重要指标.
- 优化的PSO-BP模型实现了高分类准确率84.49%的性别确定.
结论:
- 指纹中的相对氨基酸度提供了一种新的,简单且准确的性别确定方法.
- 开发的分析方法能够应对传输损失,老化时间和个体变化等因素.
- 这项研究为开发敏感氨基酸试剂提供了宝贵的参考资料,并解决了指纹残留研究中的稳定性问题.
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