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Three-Dimensional Microscopy in Microbiology

Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...

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Updated: Apr 7, 2026

An Analytical Tool that Quantifies Cellular Morphology Changes from Three-dimensional Fluorescence Images

An Analytical Tool that Quantifies Cellular Morphology Changes from Three-dimensional Fluorescence Images

Published on: August 31, 2012

FoamQuant:一个用于细胞材料的时间分辨率3D图像定量化的Python包

Florian Schott¹, Benjamin Dollet², Stéphane Santucci³

¹Division of Solid Mechanics, LTH, Lund University, Lund, Sweden.

Journal of synchrotron radiation

|August 22, 2025

概括

我们开发了一个免费的软件FoamQuant, 这个工具量化了动态泡结构中的气泡特性和机械行为.

关键词:

细胞材料图像分析断层扫描

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Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope

Published on: April 7, 2014

Automated Quantification of Hematopoietic Cell – Stromal Cell Interactions in Histological Images of Undecalcified Bone

Automated Quantification of Hematopoietic Cell – Stromal Cell Interactions in Histological Images of Undecalcified Bone

Published on: April 8, 2015

相关实验视频

Last Updated: Apr 7, 2026

An Analytical Tool that Quantifies Cellular Morphology Changes from Three-dimensional Fluorescence Images

An Analytical Tool that Quantifies Cellular Morphology Changes from Three-dimensional Fluorescence Images

Published on: August 31, 2012

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope

Published on: April 7, 2014

Automated Quantification of Hematopoietic Cell – Stromal Cell Interactions in Histological Images of Undecalcified Bone

Automated Quantification of Hematopoietic Cell – Stromal Cell Interactions in Histological Images of Undecalcified Bone

Published on: April 8, 2015

科学领域:

材料科学
物理
生物技术

背景情况:

对于3D结构分析而言,
时间分辨率X射线断层扫描的动态4D数据集带来了显著的图像分析挑战.
现有的工具难以处理4D细胞材料数据的复杂性.

研究的目的:

这是一个全新的,免费的,开源软件包.
为4D细胞或类似泡的材料提供专用平台进行批处理和定量分析.
能够详细描述随着时间的推移而演变的材料结构.

主要方法:

开发了一个模块化且易于使用的软件包FoamQuant.
实施大型4D时间序列数据集的批处理算法.
使用FoamQuant分析液体泡动力学和泡粗.

主要成果:

FoamQuant成功地提取了诸如液体分数 (孔径) 和单个气泡体积等关键参数.
实现了机械性质的高级表征,包括弹性应变和应力场.
在流动的泡中量化了单个细胞的重新排列和塑性事件.

结论:

需要专门的4D图像分析工具.
该软件有助于深入了解材料的演变,机械特性和动态过程.
通过对液体泡和粗白蛋白泡的案例研究证明了效用.