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Updated: Sep 10, 2025

Simultaneous pH Measurement in Endocytic and Cytosolic Compartments in Living Cells using Confocal Microscopy
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Simultaneous pH Measurement in Endocytic and Cytosolic Compartments in Living Cells using Confocal Microscopy

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用于可视化 lysosomal pH 波动的色度和开启光探针

Xiaofei Yin1,2, Boyang Zhang2, Yibin Wang1,2,3

  • 1Qingdao Key Laboratory of Analytical Technology Development and Offshore Eco-Environment Conservation, Marine Bioresource and Environment Research Center, First Institute of Oceanography, Ministry of Natural Resources, Qingdao 266061, China. yxf@fio.org.cn.

Analytical methods : advancing methods and applications
|August 22, 2025
PubMed
概括

研究人员开发了一种新的红至近红外光探测器来监测 lysosomal pH. 这种工具有助于在热中风等细胞应激过程中可视化pH值变化,

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Single Liposome Measurements for the Study of Proton-Pumping Membrane Enzymes Using Electrochemistry and Fluorescent Microscopy12:15

Single Liposome Measurements for the Study of Proton-Pumping Membrane Enzymes Using Electrochemistry and Fluorescent Microscopy

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Measuring Phagosome pH by Ratiometric Fluorescence Microscopy14:39

Measuring Phagosome pH by Ratiometric Fluorescence Microscopy

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相关概念视频

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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