从岛内皮层向侧面的侧臂核的下降突出促进了大鼠的恶性行为
在PubMed上查看摘要
概括
此摘要是机器生成的。岛内皮层 (IC) 到侧侧侧臂核 (LPBN) 的投射通过增加面部擦拭行为来缓解面部疼痛. 激活这些通路会在LPBN神经元中唤起更强的刺激后突触电流,从而促进疼痛缓解.
科学领域
- 神经科学
- 疼痛研究
- 身体的感知
背景情况
- 侧侧核 (LPBN) 是口面感觉的关键中继器,从三胞胎脊柱子核接收输入,并投射到像中枢桃体 (CeA) 和岛屿皮层 (IC) 这样的区域.
- 岛内皮层 (IC) 处理面部疼痛信息并向LPBN发送下降投影,这表明它在调节感知传输方面发挥了作用.
研究的目的
- 通过光遗传学来研究岛皮层 (IC) 到侧侧核 (LPBN) 的生理功能.
- 描述LPBN中的神经元亚型,参与处理来自IC的面部感知.
主要方法
- 使用光遗传刺激来激活IC→LPBN投射,并记录不同LPBN神经元亚型 (谷氨基/GABAergic,胆能/非胆能) 的唤起后突触电流 (pEPSC).
- 在素刺激后,通过Fos原基因 (c-Fos) 免疫反应来评估神经活动.
- 为了评估IC→LPBN通路激活的功能影响,进行了行为测试,包括面部擦拭和头部撤回的反射.
主要成果
- 在所有记录的LPBN神经元亚型中,IC轴突的激活引起了pEPSC.
- 在LPBN中向CeA投射的Glutamatergic神经元 (Glu-nonAch) 在IC→LPBN刺激时显示出相锁动力.
- 激活IC→LPBN预测增加了素诱导的面部擦拭行为,并降低了头部撤回反射的门,表明面部疼痛处理增强.
结论
- IC投射显著影响LPBN神经元活动,特别是在激发投射神经元中,从而促进面腔感知.
- IC→LPBN途径在面部疼痛感知和行为反应的下降调节中起着至关重要的作用.
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