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大麻素的抗生物膜活性对抗甲素耐药黄金葡萄球菌

Pancy Tsz Hei Kwong¹, Theerthankar Das², Jonathon Carl Arnold³

¹Advanced Drug Delivery Group, Sydney Pharmacy School, Faculty of Medicine and Health, The University of Sydney, Sydney, NSW 2006, Australia.

Journal of applied microbiology

|August 22, 2025

概括

大麻素对抗甲素耐药黄金葡萄球菌 (MRSA) 生物膜具有潜力. 大麻素 (CBN) 显示出最强的抗生物膜活性,而大麻素 (CBD) 在MRSA中引起显著的膜损伤.

科学领域:

微生物学
药理学
自然产品化学

背景情况:

甲素耐药黄金葡萄球菌 (MRSA) 作为医院获得的肺炎的原因构成重大威胁.
由于耐药性机制,现有的抗生素往往对MRSA无效.
新的治疗策略,包括对大麻素等天然化合物的探索,对于对抗MRSA感染至关重要.

研究的目的:

评估五种主要植物性大麻素对MRSA的抗生物膜活性.
为了比较大麻 (CBD),delta-9-tetrahydrocannabinol (THC),cannabinol (CBN),cannabigerol (CBG) 和cannabichromene (CBC) 在抑制MRSA生物膜中的功效.
研究潜在的作用机制,包括对细菌活力,代谢活性,活性氧物种 (ROS) 生产和膜完整性的影响.

主要方法:

使用晶体紫色染色和resazurin代谢测试来评估抗生物膜活性.
测量细胞内活性氧物种 (ROS) 含量和酸吸收量,以评估膜完整性.
确定每个大麻素对MRSA的最小抑制度 (MIC).

主要成果:

所有测试中的大麻素都显示出1~2μg/ml之间的最小抑制度.
大麻素 (CBN) 显示出最强的抗MRSA生物膜活性,显著降低生物膜生物量和细菌活力.
大麻二醇 (CBD) 在减少生物膜生物量方面最不有效,但在MRSA生物膜中诱导了最显著的膜损伤.

结论:

植物性大麻素具有不同程度的抗MRSA生物膜活性和可能不同的作用机制.
大麻素和大麻二醇对MRSA生物膜有不同的作用,强调需要进一步的机制研究.
这些发现表明大麻素是新型抗MRSA生物膜疗法的潜在候选者,需要进一步研究.

关键词:

黄金葡萄球菌抗菌药物生物膜大麻素反应性氧物种电阻

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