尿酶介导微生物凝固的操作值:在周围地下水环境中进行多变量优化和现场验证
Jiajian Bao1, Wangqing Xu1,2, Dongming Zhou1
1School of Civil Engineering and Architecture, Hubei University of Arts and Science, Xiangyang, Hubei, China.
PloS one
|August 22, 2025
概括
这项研究优化了尿酶抑制的微生物诱导碳酸沉 (MICP) 以实现可持续的土壤稳定. 它为环境条件下N-n-butyl-thiophosphoric triamide (NBPT) 的应用设定了实际门,提高了成本效益和环境兼容性.
科学领域:
- 地质工程
- 环境科学
- 微生物学
背景情况:
- 微生物诱导碳酸沉 (MICP) 提供可持续的土壤稳定性,但面临反应动力学和环境控制方面的挑战.
- 现有的MICP方法通常需要能源密集的条件,限制了实际的大规模应用.
研究的目的:
- 开发使用N-n-butyl) -thiophosphoric triamide (NBPT) 的尿素酶抑制MICP的多变量优化框架.
- 在环境地下水条件下确定MICP的实际应用值,重点是成本效益和环境兼容性.
主要方法:
- 对五个操作参数进行系统的调查:NBPT度,凝固溶液度,细菌与水泥溶液的比率,温度和pH值.
- 使用沙柱测试和持续的尿酶活动监测来评估性能.
- 评估未受限制的压力强度以确定有效的生物凝固.
主要成果:
- 低NBPT度 (0.1%) 和1:1的细菌悬浮与凝固溶液的比率实现了有效的生物凝固.
- 与没有抑制剂的对照组相比,保持了93%的未受限制的压力强度.
- 确定了最佳条件:1mol/L凝固溶液,温度大于20°C,pH值为中性 (7-8).
结论:
- 该研究为NBPT-MICP在地质技术应用,桥梁实验室优化和现实世界的可变性提供了实用,可实施的指导方针.
- NBPT-MICP被推广为天然水化系统中具有成本效益和环境兼容的沙稳定解决方案.
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