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相关概念视频

Maintenance of the ES Cell State01:14

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The cells of the blastocyst inner cell mass only remain pluripotent for a short time. This state of pluripotency and self-renewal can be maintained in embryonic stem (ES) cell culture by adding specific chemicals or growth factors to ensure the cells can continue dividing and later differentiate into different cell types. In some cases, the cells are grown on a feeder layer of differentiated cells, which provides the growth factors and extracellular matrix components necessary for stem cell...
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Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
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Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...
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Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
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在RSeT人类胚胎干细胞中抗原和形成性多能性转化

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人类胚胎干细胞 (hESC) 代表一种独特的多能状态,与天真和原始状态不同. 这些细胞表现出独特的生长特性,缺乏关键的多能性标记,为干细胞状态过渡提供了新的见解.

关键词:
在 RSeT 中介细胞增殖人类胚胎干细胞纯粹的多能性转录组

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科学领域:

  • 干细胞生物学
  • 发育生物学
  • 基因组学

背景情况:

  • 人类胚胎干细胞 (hESC) 呈现纯粹和原始化的多能状态.
  • 之前的工作从各种协议中确定了异质的天真多能状态.
  • 了解多能状态异质性对于干细胞应用至关重要.

研究的目的:

  • 描述一个基于RSeT的商业多能状态.
  • 在不同的生长条件下研究RSeT hESC的行为.
  • 定义RSeT hESC与其他多能状态相关的转录和生化特性.

主要方法:

  • 在正常和低氧条件下培养细胞.
  • 单细胞涂层效率测试
  • 整合性转录组分析
  • 表面标记表达的分析 (SUSD2,CD75).
  • 对信号通路依赖的生物化学测试 (FGF2,JAK,TGFβ).

主要成果:

  • RSeT hESC可以在没有低氧的情况下生长,但表现出可变的生长和涂层效率.
  • RSeT hPSC 缺乏原始和形成性多能性的转录学特征.
  • RSeT hESC 类似于早期植入后胚胎,类似于初级 hESC.
  • 原始表面标记SUSD2和CD75没有显著表达.
  • RSeT hESC对FGF2表现出细胞系特异性的依赖性和对JAK/TGFβ信号的共依赖性.

结论:

  • RSeT hESCs代表了天真多能性的下游新型多能状态.
  • 通过维持FGF2活动,RSeT介质可能会限制纯粹的多能潜能.
  • 这项研究增强了对体外多能性转变的理解.