相关实验视频

Updated: Sep 10, 2025

Optimized Negative Staining: a High-throughput Protocol for Examining Small and Asymmetric Protein Structure by Electron Microscopy

Optimized Negative Staining: a High-throughput Protocol for Examining Small and Asymmetric Protein Structure by Electron Microscopy

Published on: August 15, 2014

含有低密度脂蛋白的阿波脂蛋白的结构,功能和生化表征

Yubexi Correa¹, Favour Nzekwe¹, Tigist Wodaje²

¹Biofilm - Research Center for Biointerfaces and Department of Biomedical Science, Faculty of Health and Society, Malmö University, 20506 Malmö, Sweden.

Journal of colloid and interface science

|August 22, 2025

概括

低密度脂蛋白 (LDL) 颗粒中高水平的脂蛋白 (Lp) 可能会损害它们的功能. 小密度的LDL分片的结构差异,而不是总的LDL,可能解释了动脉样硬化的这种功能障碍.

科学领域:

心血管研究
生物化学
结构生物学

背景情况:

动脉样硬化是全球的主要健康问题,低密度脂蛋白 (LDL) 起着关键作用.
脂蛋白 (Lp) 是一种LDL变体,是动脉样硬化的独立预测因子.
由于其非脂蛋白成分,Lp (a) 具有独特的特性,但在结构上仍未得到充分研究.

研究的目的:

在生物化学,结构和功能上描述具有不同Lp (a) 水平的LDL颗粒.
调查低Lp和高Lp之间的潜在结构差异.
探索Lp (a) 丰度在LDL分数中的作用及其功能影响.

主要方法:

从低Lp或高Lp的正常脂质体中分离LDL颗粒.
福里埃变换红外光谱 (FTIR) 用于脂质去除的评估.
用于结构分析的小角度X射线散射 (SAXS).
在LDL分数中的Lp (a) 丰度的西方斑点分析.

主要成果:

与低Lp(a) LDL相比,高Lp(a) LDL显示出模型膜的脂质去除减少.
在总LDL分数之间观察到核心与外散射质量比的显著差异.
在小密度的LDL (LDL₆) 分数中较为丰富.
来自高Lp (a) 个体的LDL₆分片显示蛋白质外厚度增加.

结论:

与Lp (a) 水平相关的LDL的功能差异可能不仅仅由总的LDL结构来解释.
结构变化,特别是LDL₆的蛋白质外厚度增加,与高Lp(a) 水平有关.
小密度的LDL₆部分在升高Lp的背景下对调解LDL功能障碍显得至关重要.

动脉样硬化心血管疾病脂肪蛋白 (a)低密度脂蛋白微角X射线散射结构功能

更多相关视频

Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein

Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein

Published on: October 12, 2017

Isolation and Analysis of Plasma Lipoproteins by Ultracentrifugation

Isolation and Analysis of Plasma Lipoproteins by Ultracentrifugation

Published on: January 28, 2021

相关概念视频

Receptor-mediated Endocytosis

Receptor-mediated Endocytosis

Lipid-derived Compounds in the Human Body

Lipid-derived Compounds in the Human Body

Fats and lipids are crucial components in the human body. Some lipid-derived compounds, such as fat-soluble vitamins, eicosanoids, lipoproteins, and glycolipids, also play unique roles to support various biological processes .
Fat-soluble Vitamins
Fat-soluble vitamins, including vitamins A, D, E, and K, are required in minimal quantities, but their deficiencies can lead to severely abnormal physiological conditions. For example, vitamin A deficiency can cause night blindness, dry skin,...

Atherosclerosis I: Introduction

Atherosclerosis I: Introduction

Atherosclerosis is a progressive disorder characterized by the buildup of plaques on the arterial inner wall, causing them to narrow and harden over time. These plaques comprise lipids, calcium, blood components, carbohydrates, and fibrous tissue. The process primarily affects the intima of large and medium-sized arteries, reducing blood flow in any artery.Etiology and risk factorsThe cause of atherosclerosis is multifactorial, involving a complex interplay among endothelial injury, lipid...

Cholesterol: Significance and Regulation

Cholesterol: Significance and Regulation

Although not a source of energy, cholesterol plays a significant role as a foundational structure for bile salts, steroid hormones, and vitamin D, as well as being a crucial component of plasma membranes. Approximately 15% of blood cholesterol is derived from our diet, with the remainder synthesized from acetyl CoA by the liver and intestines. Cholesterol is eliminated from the body through its conversion into bile salts, which are eventually discarded in the feces.
Considering cholesterol and...

Asymmetric Lipid Bilayer

Asymmetric Lipid Bilayer

Biological membranes show uneven distribution of different types of lipids in the inner and outer layers, resulting in transverse asymmetric membranes. The treatment of the erythrocyte membrane with the enzyme phospholipase confirmed the asymmetric nature of the lipid bilayer. The enzyme hydrolyzes lipids into fatty acids and hydrophilic groups. The phospholipase acts only on the outer layer of the membrane, while the inner layer remains intact. The phospholipase treatment resulted in 80%...

Blood Studies for Cardiovascular System III: Serum Lipid Profile

Understanding serum lipids is crucial for maintaining cardiovascular health and preventing heart disease and stroke.
Serum lipids are fats and fatty substances in the blood and are crucial for various bodily functions, including energy storage, cellular structure, and hormone production. Serum lipids consist of cholesterol, triglycerides, and phospholipids.
Cholesterol is a soft, fat-like substance found in all body cells. It is crucial for producing hormones, vitamin D, and substances that aid...