拉曼光谱是一种通过真空包装羊肉的包装材料预测可活性的微生物总数的非侵入性方法
Benjamin W B Holman1, David L Hopkins2, Stephanie M Fowler3
1Wagga Wagga Agricultural Institute, NSW Department of Primary Industries and Regional Development, Wagga Wagga, New South Wales, 2650, Australia; Gulbali Institute, Charles Sturt University, Wagga Wagga, 2678, Australia.
Meat science
|August 22, 2025
概括
拉曼光谱为评估冷羊肉中的微生物水平提供了一种非破坏性的方法. 虽然总活力计数 (TVC) 的直接预测很简单,但它可以准确地区分高和低微生物污染.
科学领域:
- 食品科学与技术
- 分析化学
- 微生物学
背景情况:
- 监测冷肉中的微生物污染对于食品安全和质量至关重要.
- 传统的可行性计数 (TVC) 方法具有破坏性和耗时性.
- 需要使用非破坏性技术快速评估包装肉类中的微生物负载.
研究的目的:
- 调查拉曼光谱法在真空包装的冷却羊肉中预测微生物的总活力计数 (TVC) 的有效性.
- 评估拉曼光谱法在羊肉中区分高低TVC水平的能力.
主要方法:
- 在真空包装中对159只羊的长腰肌肉 (LL) 进行了拉曼光谱检查.
- 使用785nm激光器的手持式拉曼光谱仪进行光谱采集.
- 随后的分析包括从包装中取出样本以确定TVC并与光谱数据进行比较.
主要成果:
- 拉曼光谱对包装中的TVC具有适度的预测能力 (R2=0. 29;RMSE=1. 34).
- 然而,拉曼光谱成功地区分了高和低的TVC水平 (基于log 5 CFU/g) 的高精度 (92.5%) 和灵敏度 (88.0%).
结论:
- 拉曼光谱可以提供有关冷藏羊肉的微生物状况的有价值的非破坏性信息.
- 这项技术对包装肉制品中的微生物负载进行了快速的非侵入性监测.
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