全基因组的CRISPR选识别了提高CAR-NK细胞抗瘤功能的关键标

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Updated: Sep 10, 2025

Performing an In Vitro Genome-Wide CRISPR Knockout Screen in Chimeric Antigen Receptor T Cells
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Performing an In Vitro Genome-Wide CRISPR Knockout Screen in Chimeric Antigen Receptor T Cells

Published on: January 31, 2025

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Alexander Biederstädt1, Rafet Basar2, Jeong-Min Park2

  • 1Department of Stem Cell Transplantation and Cellular Therapy, The University of Texas MD Anderson Cancer Center, Houston, TX, USA; Institute for Cell Therapy Discovery and Innovation, The University of Texas MD Anderson Cancer Center, Houston, TX, USA; Department of Medicine III: Hematology & Oncology, School of Medicine, Technical University of Munich, Munich, Germany.

Cancer cell
|August 22, 2025
PubMed
概括

人工合成的自然杀手 (NK) 细胞对癌症治疗具有前景. 基因编辑确定了MED12,ARIH2和CCNC作为增强NK细胞抗瘤活性和克服免疫抑制的点.

关键词:
CAR-NK细胞疗法采用细胞治疗功能性扰动力学全基因组CRISPR选代谢重编程复合细胞工程自然杀手细胞精确基因编辑固体瘤瘤微环境

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CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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