单细胞转录组分析突出了菌头部菌操纵的特定细胞类型,导致小麦易受感染
Wan-Qian Wei1, Shuang Li2, Dong Zhang3
1State Key Laboratory of Plant Trait Design, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai 200032, China; University of Chinese Academy of Sciences, Beijing 100049, China; Key Laboratory of Birth Defects and Related Diseases of Women and Children of Ministry of Education, West China Second Hospital, Sichuan University, Chengdu 610041, Sichuan, China.
Developmental cell
|August 22, 2025
概括
小麦植物使用细胞特异性防御来对抗真菌入侵者. 单细胞RNA测序揭示了适应的Fusarium graminearum (Fgr) 与非适应的Fusarium oxysporum (Foc) 的不同免疫反应.
科学领域:
- 植物病理学
- 分子生物学
- 基因组学
背景情况:
- 植物拥有免疫系统来检测和防御入侵的真菌.
- 病原体的适应可以导致植物的易感性,并复杂化抗病策略.
- 多细胞植物可能使用细胞分化的反应来管理真菌相互作用.
研究的目的:
- 在感染适应型 (Fusarium graminearum) 和非适应型 (Fusarium oxysporum f. sp.) 的小麦类动物中研究细胞类型特异性转录基因反应. 这种真菌.
- 在单细胞分辨率下阐明植物病原体相互作用的分子机制.
主要方法:
- 使用单细胞RNA测序 (scRNA-seq) 来分析小麦类动物的9万多个细胞.
- 用Fgr和Foc在注射后的1,2,3天进行了转录组分析.
- 对八种主要细胞类型进行了差异基因表达和伪时间轨迹分析.
主要成果:
- 在小麦类动物中确定了细胞类型特异性免疫反应.
- 在对非适应的Foc (非宿主耐药性) 反应中观察到分隔免疫通路的上调.
- 检测到广泛的免疫通路激活响应适应Fgr (敏感相互作用) 和Fgr诱导的基细胞活动.
结论:
- 小麦类动物对适应和非适应的真菌病原体表现出不同的细胞防御策略.
- 单细胞分辨率显示了各种细胞类型的免疫反应的差异调节.
- Fusarium graminearum感染导致特定植物细胞的转录活动抑制,导致疾病易感性.
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