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在选和实验验证中确定里波弗拉是针对SARS-CoV-2的RNA抗病毒药物

Chae-Hong Jeong¹, Yoo Jin Na¹, Tae Yong Kim¹

¹Division of Clinical Research, Center for Emerging Virus Research, National Institute of Infectious Diseases, Korea National Institute of Health, 212 Osongsaengmyeong2-ro, Osong-eup, Heungdeok-gu, Cheongju, 28160, Republic of Korea.

Scientific reports

|August 22, 2025

概括

这项研究将利博弗拉重新用作针对SARS-CoV-2的RNA抗病毒药物. 在接种期间,黄素通过抑制病毒复制表现出选择性抗病毒活性.

科学领域:

病毒学
药物发现
计算生物学

背景情况:

SARS-CoV-2 突变对抗病毒疗法构成挑战.
针对保存的病毒RNA结构提供了对抗病毒演变的策略.
药物重新使用可以加速新疗法的开发.

研究的目的:

识别潜在的药物重用候选者,针对保存的SARS-CoV-2RNA结构.
通过计算选化合物与这些RNA元素结合.
通过实验验证有前途的抗病毒活性.

主要方法:

对283个SARS-CoV-2基因组进行生物信息分析以确定保存的RNA元素.
使用RNAfold和RNAstructure进行RNA二次结构预测.
使用RNALigands数据库对11种化合物的虚拟选.
在体外验证Vero E6细胞中的抗病毒活性和细胞毒性.

主要成果:

利博弗拉对SARS-CoV-2具有选择性抗病毒活性 (IC50 = 59.41μM),没有观察到100μM以下的细胞毒性.
病毒接种期间的 riboflavin 治疗显著减少了病毒复制.
其他查的化合物缺乏显著的抗病毒功效.
利博弗拉文的强度低于雷梅西维尔 (IC50 = 25. 81 μM).

结论:

利博弗拉是一种潜在的RNA向治疗剂,可对抗SARS-CoV-2.
该研究框架整合了RNA病毒标识的计算和实验方法.
利博弗拉可以作为广泛的抗病毒药物,强调营养因素在病毒感染中的作用.

关键词:

抗逆转录病毒药物重新使用在中 RNA二次结构利博弗拉冠状病毒

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