WWTP产品修改对Phaseolus vulgaris根球的影响及其使克拉里素无活化的能力
Lucie Kotrbová1,2, Kateřina Grabicová3, Helena Švecová3
1Department of Ecosystem, Faculty of Science, University of South Bohemia, České Budějovice, Czech Republic.
Scientific reports
|August 22, 2025
概括
农业废水产品可能会增加抗生素耐药性的细菌. 然而,特定的土壤微生物,特别是菌体,可以降解单素 (CLR),这表明生物修复策略的潜力.
科学领域:
- 环境微生物学
- 土壤科学
- 农业科学
背景情况:
- 废水在农业中的再利用正在增加, 引发土壤中抗生素残留的担忧.
- 克拉里素 (CLR) 是污水中常见的抗生素,其对土壤微生物群落的影响需要调查.
- 了解植物相关微生物的降解潜力对于评估抗生素残留的风险至关重要.
研究的目的:
- 调查植物相关微生物的清红素 (CLR) 降解潜力.
- 评估废水处理厂 (WWTP) 产品对土壤微生物群落和CLR耐药性的影响.
- 确定能够降解农业土壤中CLR的微生物群.
主要方法:
- 植物在用WWTP产品 (废水,生物固体,堆肥生物固体) 修改的土壤中生长.
- 使用16S rRNA转基因组学和培养技术来分析根球微生物组.
- 使用微生物联合体,丰富培养,抗生素调节和LC-MS进行体外测定,以评估CLR降解.
主要成果:
- 生物固体的修订丰富了树根层的Pseudomonadaceae;CLR丰富培养是由蛋白质细菌主导的.
- 没有观察到初始微生物联合体或丰富培养的CLR降解,这表明耐药细菌的增殖.
- 来自七个基因组的actinomycetes在体外显示完全消除了CLR.
- 通过WWTP产品的修订,增加了土壤中CLR无活化的微生物的数量和多样性.
结论:
- 根球中含有CLR无活化的微生物,其中的菌体具有显著的降解潜力.
- 虽然WWTP的修订可能会增加耐CLR的细菌,但它们也会增加有益的CLR降解微生物的种群.
- 这突显了利用回收水资源的农业系统中抗生素残留物,土壤微生物和植物健康之间的复杂相互作用.
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