Maspin/SerpinB5是一种细胞骨结合蛋白,可以调节上皮细胞的形状
Luiz E da Silva1, Lia M G Paim2, Tatiana Lyalina2
1Department of Cell and Developmental Biology, University of São Paulo, São Paulo, Brazil.
Communications biology
|August 22, 2025
概括
Maspin (SerpinB5) 与细胞骨组成部分相互作用,影响细胞形状和粘附. 这种蛋白调节细胞与细胞的接触和细胞骨的重组,影响细胞迁移和分裂.
科学领域:
- 细胞生物学
- 分子生物学
- 生物化学
背景情况:
- 马斯 (SerpinB5) 是一种丰富的蛋白质,主要通过表皮表达.
- 最初被认为是一种瘤抑制剂,Maspin调节细胞粘附,迁移和入侵.
- 马斯宾调节这些细胞过程的细胞内机制在很大程度上尚不清楚.
研究的目的:
- 阐明Maspin在调节细胞骨动力学和细胞形态学的细胞内功能.
- 为了确定Maspin在细胞内的直接蛋白相互作用伙伴.
主要方法:
- afinity 净化 - 质谱 (AP/MS) 用于识别与 Maspin 相互作用的蛋白质.
- 用于本地化研究的内源Maspin的CRISPR/Cas9介导的GFP标记.
- 在Maspin枯竭实验中使用RNA干扰 (RNAi) 和CRISPR/Cas9.
- 在体外微管聚合试验.
主要成果:
- 马斯直接与微管和微纤维相互作用.
- 内源性Maspin局部化到皮质细胞骨和线粒体.
- 马斯宾耗尽改变了细胞与细胞的接触,诱导了细胞骨的重组,并影响了细胞在分裂过程中的形状.
- 在体外和细胞内,Maspin抑制了微管的生长.
结论:
- 马斯在细胞皮层和线粒状中发挥动态作用,与细胞骨元素相互作用.
- 马斯调节细胞粘附,细胞形状和细胞骨组织.
- 这些发现揭示了Maspin在上皮细胞生物学中的新型细胞内功能.
相关概念视频
01:02Role of Septins
1.8K
Septins are the recently discovered fourth major protein component of the cytoskeleton, along with microfilaments, microtubules, and intermediate filaments. These proteins can associate with other cytoskeletal filaments and carry out varied roles or can be free-floating in the cytoplasm.
Cellular Functions of Septins
Recent studies have revealed the multifaceted roles of septins in various cellular processes such as cytokinesis, ciliogenesis, and neurogenesis. Septins act as scaffolds and...
Cellular Functions of Septins
Recent studies have revealed the multifaceted roles of septins in various cellular processes such as cytokinesis, ciliogenesis, and neurogenesis. Septins act as scaffolds and...
1.8K
01:19Septins
1.9K
Septins are protein filaments forming the cytoskeleton along with the microtubules, microfilaments, intermediate filaments, and other accessory proteins. In 1971 while studying the cell division cycle in mutant Saccharomyces cerevisiae Harwell et al. first identified the septin-related genes playing a crucial role in yeast cytokinesis. Fluorescence microscopy revealed that these proteins localize at the budding neck as rings. These ring-like proteins were then named Septins by John Pringle, and...
1.9K
01:32Cytoskeletal Coordination in Cell Migration
4.9K
A migrating cell changes its shape during the cyclic events of attachment and detachment from the substratum and repositions the cell organelles correspondingly. These complex events are orchestrated by the dynamic cytoskeletal network comprising actin filaments, intermediate filaments, and microtubules. Cytoskeletal crosstalk — the direct and indirect communication between the different components — is crucial for this coordination. Direct communication involves various linker...
4.9K
01:39Mechanism of Filopodia Formation
2.5K
Filopodia are thin, actin-rich cellular protrusions that play an important role in many fundamental cellular functions. They vary in their occurrence, length, and positioning in different cell types, suggesting their diverse roles.
Their main function is to guide migrating cells during normal tissue morphogenesis or cancer metastasis by recognizing and making initial contacts with the extracellular matrix. However, they can also act as stationary cell anchors or help to establish communication...
Their main function is to guide migrating cells during normal tissue morphogenesis or cancer metastasis by recognizing and making initial contacts with the extracellular matrix. However, they can also act as stationary cell anchors or help to establish communication...
2.5K
01:32Assembly of Complex Microtubule Structures
1.9K
Complex microtubule structures are present in resting cells and in dividing cells. In resting cells, they are responsible for maintaining the cellular architecture, tracks for intracellular transport, positioning of organelles, assembly of cilia and flagella. They mediate the bipolar spindle assembly for chromosomal segregation and positioning of the cell division plate in dividing cells. The formation of microtubule complex structures depends on the cell type, cell stage, and cell function.
1.9K
01:13Cytoskeletal Accessory Proteins
3.2K
The cytoskeleton is an essential cell component that plays several structural and functional roles. However, the filaments that make up the cytoskeleton cannot function independently and depend on the accessory or ancillary proteins to effectively carry out their function. Accessory proteins associate with cytoskeletal filaments and their monomers, aiding filament formation and function. They also help in the cross-communication among cytoskeletal filaments. Cytoskeletal accessory proteins are...
3.2K