佩里拉精油:一种天然的抗菌剂
Hai-Run Fan1, Man Hao2, Jie Yang1
1School of Food Science and Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan, China.
Journal of the science of food and agriculture
|August 23, 2025
概括
佩里拉精油 (PEO) 有效地对抗Listeria monocytogenes,这是一个不断增长的食源性病原体. 这种天然替代物破坏细菌细胞结构,减少生物膜的形成,为食品安全提供了有希望的解决方案.
科学领域:
- 食品科学
- 微生物学
- 自然产品化学
背景情况:
- 由Listeria monocytogenes引起的食物感染的发生率上升.
- 由于健康风险,需要使用天然抗菌剂替代化学剂.
- 对Perilla精油 (PEO) 对L. monocytogenes的疗效进行的研究有限.
研究的目的:
- 研究Perilla精油 (PEO) 对Listeria monocytogenes的抗菌特性.
- 评估PEO作为天然抗菌剂在食品安全方面的潜力.
主要方法:
- 通过水蒸从干燥的叶中提取PEO.
- 使用气色谱-质谱法 (GC-MS) 识别PEO化合物.
- 通过MIC/MBC测定,SEM成像,表面疏水性测量,生物膜生物质量量测量,核酸/蛋白质泄漏测定,凝电泳和呼吸链脱酶活性测定来评估抗菌活性.
主要成果:
- PEO对L. monocytogenes具有显著的抗菌活性,MIC/MBC值为1/4毫克/毫升.
- SEM显示PEO诱导的细菌细胞变形和破裂.
- PEO增加了细菌表面的疏水性,减少了生物膜的形成,并破坏了细胞壁/膜,导致细胞内容的泄漏.
- PEO治疗显著降低了细菌蛋白质含量和呼吸链脱酶活性.
结论:
- 佩里拉精油对Listeria monocytogenes具有强大的抗菌作用.
- 作为控制食品中L. monocytogenes污染的天然抗菌剂,PEO具有前景.
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